FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072616
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA28290.26374769721876234No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA25060.23363440411107048No Hit
AGCACAGCCACTATGCTTGATTAGCATTACAGAGATGGTACAATGATGGT24670.22999843373583836No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG24600.229345823668489No Hit
AAATATAGTCATAACATTGTGCAGCCGTCCCTACCATCCATCTCCATAAT22240.2073435413978535No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA21850.2037075710226213No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT21780.20305496095527198No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC20450.19065536967563415No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA20160.18795169939661538No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG20070.18711262931002334No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC19960.18608709920418862No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC19590.18263758884819917No Hit
ATTATGTTCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGC17460.1627795967988544No Hit
TTGTCCAGCCGTCCCTACCATCCATCTCCATAATTCGTTTCATCTTGTAA15640.14581173504777106No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT14830.13826010426844276No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC14780.13779395422033608No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT14520.1353699739701813No Hit
AACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGC14300.1333189137585119No Hit
CTATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGA14190.1322933836526772No Hit
CTGCATATCTGCTCCTTTGTATAAAGGATAAACAAAAACTTTAAAGTACT13900.1295897133736584No Hit
GTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTGCTCCT13830.12893710330630906No Hit
GTATTTTTTAATGGGTATAGAGTTTCAGTTTTACAAGATGAAACGAATTA13420.12511467291183423No Hit
GTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGG13140.12250423264243682No Hit
GTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTAGG12590.11737658211326328No Hit
CACTTGGTCCATGGGGCTCCGCTTCCTTCCCATGGCCTCCCCTCTGGGGT12560.11709689208439927No Hit
AGCACAGCCACTATGCTTGATTAGCATTATAGAGATGGTACGATGATGGT11600.10814681116075092No Hit
TCTCTCTTCTGTCCTTCCAAGCCTACTAAGCACGTGGCATCCTCCCAGAT11410.1063754409779455No Hit
ACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGAT10980.10236655056422803No Hit
GTACAATGATGGTTGCCAGAAGCTGGGGGGAGGAAGAAATGGGGAAGTAT10810.1007816404006653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGCG256.465234E-6115.999479
GTAACAA35750.0113.363134
TAGTAAC40900.099.7978442
AGCACGC8100.099.351411
GCCGACC607.2759576E-1296.66623145
GCACGCA8750.091.971012
AGTAACA44400.091.76763
TAACAAG45000.090.544045
CGCACTT8850.082.739745
ACGCACT11000.082.385994
GAAACAC12450.082.108064
ACAAGAG49300.081.911397
CACGCAC10050.080.074273
GTAGTAA52550.078.363111
CAAGAGC43600.076.823058
GTCTCGC2600.075.2881241
TGACGCC2450.071.02009145
AACAAGA57800.070.24196
CGGTGCA5700.068.68392
GTAGAAA22150.068.080961
Produced by FastQC (version 0.11.5)