FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1904188
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA75090.394341315038221No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC46380.2435683871550498No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA43370.22776112442678978No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT40180.2110085768842152No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG38000.19956012746640564No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT36120.1896871527391203No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC33880.17792360838320584No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG32540.1708864880988642No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC31820.167105348841606No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA30210.15865030133579247No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT29840.1567072158841459No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC29500.15492167790155173No Hit
ATTATGTTCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGC27590.144891155705214No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC26070.13690875060655777No Hit
AAATATAGTCATAACATTGTGCAGCCGTCCCTACCATCCATCTCCATAAT24960.13107949425161802No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA24900.13076439931351316No Hit
GTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGG22230.1167426745678473No Hit
AACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGC21800.11448449417809586No Hit
CTGCATATCTGCTCCTTTGTATAAAGGATAAACAAAAACTTTAAAGTACT21360.11217379796532696No Hit
CTCTTGACCATTTTAAGTGTACAGTTCAGTGGTACTAAATAGAGTCATAA21310.11191121885023958No Hit
GTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTAGG21300.1118587030272221No Hit
CTATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGA21170.11117599732799492No Hit
GTAGTAACAAGAGCAAGTTGCTTAGCTTCTCAGAGCCTTGCCAAACCCCA19360.10167063336183192No Hit
AGCACGCACTTTACATACATGTTATTTCCCTTCACAGTAACCCCTGGGAC19360.10167063336183192No Hit
AGCACAGCCACTATGCTTGATTAGCATTATAGAGATGGTACAATGATGGT19250.1010929593086397No Hit
GTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTGCTCCT19060.10009515867130767No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGCG100.0070914384144.999474
GCACGCA14800.0113.158382
AGCACGC14900.0112.3989261
GTAACAA70500.0106.435784
TAGTAAC72900.0103.428982
TAACAAG73200.0102.311795
CGCACTT16850.096.379485
ACGCACT19150.095.0257644
AGTAACA81050.092.939213
CACGCAC18100.092.527293
GCCGACC1250.086.99969145
CGGTGCA5100.085.293812
ACAAGAG92450.081.16527
GTCTCGC6900.079.854781
AAACACG22500.078.944155
GTAGTAA97100.078.622281
CGCACTG2100.075.95215
GAAACAC24000.074.614324
CTCGCTC7300.072.499743
CGAGCAC1600.072.499733
Produced by FastQC (version 0.11.5)