FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_105.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_105.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2774142
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC127850.46086321464438373No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT108020.3893816538591031No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT72580.2616304428540428No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT70080.2526186474953337No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC69520.25060000533498283No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA61790.2227355340858543No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG54910.19793507325868684No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA51840.18686858855819205No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA47170.17003455482812344No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC40310.14530618836382564No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT40120.14462129191656375No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG37590.13550135501355012No Hit
AGTAGAAACAACGCACTTTTTCCCAGTTTATTTGCTGACATTGATTACAA37420.1348885529291579No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA33210.11971268954509177No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA32010.1153870277729114No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA31660.11412537642269213No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA31410.11322419688682123No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC30650.11048461109777366No Hit
GTAAGAAGTACAGCAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA30070.10839387457455316No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC30040.10828573303024863No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT29580.10662756268424615No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATGT28670.10334726917367604No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC27820.10028325875171494No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT27780.10013907002597561No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC22200.0125.404981
GCACGCA23200.0119.6870962
CACGCAC23900.0115.878273
CGGTGCA5350.0102.990312
GAAACAC20700.0101.219474
TAACAAG77200.099.640215
AAACACG21900.096.33535
ACGCACT36100.088.5661854
CGCACTT36050.088.08575
ACGTGCA11550.087.8784942
GTAACAA92200.083.587034
TGACGCC1800.080.55528145
GTAGTAA101200.077.8727651
GCGGTGC14200.074.031441
GCCGACC1100.072.499756145
AACACGT7200.072.4997566
TAGTAAC109600.070.846022
AACACGA23400.068.471996
AGTAACA114950.067.674843
ACGAGCG651.399003E-766.922859
Produced by FastQC (version 0.11.5)