FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2947
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA80.2714625042416016No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC70.23752969121140144No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT70.23752969121140144No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC50.16966406515100102No Hit
GTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTCTTAGTGGAG40.1357312521208008No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA40.1357312521208008No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT40.1357312521208008No Hit
GCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG40.1357312521208008No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA40.1357312521208008No Hit
ACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGAT40.1357312521208008No Hit
GGAATGCACATGCGGATTTGCCAGCAACAAAACCATAGAATGTGCTTGTA30.10179843909060061No Hit
TTGATAGACAATTGTTCCTGTTTTCCCAGGTTTTTGCATCATGTAATCAA30.10179843909060061No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC30.10179843909060061No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG30.10179843909060061No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATGT30.10179843909060061No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC30.10179843909060061No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT30.10179843909060061No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC30.10179843909060061No Hit
ACTCAACTGCTGCCTCCAGTTTGGCTGTAACACTGATGATAGCTATCTTT30.10179843909060061No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT30.10179843909060061No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT30.10179843909060061No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTCT100.006732901145.04
AAAAAAA1653.036912E-711.424243130-134
Produced by FastQC (version 0.11.5)