FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n02_FluB_100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n02_FluB_100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2918933
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC132600.4542755863186993No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT82790.28363103915026483No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC76970.26369224644758893No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT51490.17640007495889765No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA46560.15951034162140754No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA45540.15601591403434065No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG42860.14683447684479226No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA42170.14447059935942347No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT39440.13511786669992082No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA38730.13268547102657033No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT36190.12398366115289389No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA36100.12367532930697622No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA34360.11771424695256794No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC31420.10764207331925742No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA31340.1073680005673306No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA29620.10147543640090402No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC29410.10075599542709614No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGCG100.00709184144.998055
GTAACAA58150.0123.179764
GCACGCA6050.0116.2381062
AGCACGC6000.0115.998441
CACGCAC6400.0111.014133
GAAACAC18400.0110.718614
TAACAAG68400.0104.4028245
AAACACG19650.0103.675455
CGGTGCA4500.098.276452
ACGTGCA12150.093.085172
TAGTAAC78950.091.369892
AGTAACA89900.080.240863
TAGAAAC29750.078.7132342
ACAAGAG96400.074.078367
AACACGT4800.072.499026
AACACGA23550.072.037256
CGCACTT10850.070.160355
ACGCACT11350.068.9858554
ACACGAG25750.065.882617
GAAGCGG6700.064.92451
Produced by FastQC (version 0.11.5)