FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10505371
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA38350.080.979152
CACGCAC40800.075.772793
TAACAAG244150.058.2812275
TAGTAAC248000.057.9195442
CGCACTT63450.051.8120275
ACGCACT69450.050.969284
GTCTCGC9550.049.190911
GTAACAA301800.047.4270254
GTAGTAA320550.045.4968151
ACAAGAG362900.039.1839267
AACAAGA368150.038.6696626
CTCGCTC12450.036.599813
AGCACGC106050.030.2808631
CGTGCAC10900.030.2277763
AGTAACA490350.029.693763
AACAACG51050.028.8294567
ACAACGC51300.028.5523478
AAACACG157550.026.6055435
CAAGAGC453150.025.7350048
TCGCTCT17600.025.489234
Produced by FastQC (version 0.11.5)