FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_SMART_NEGCONTROL_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_SMART_NEGCONTROL_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2189
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT341.5532206486980358No Hit
TCATTACATTGTGTCGTCACAAGTCAATTTTGAGTTTTTATATAAATAAG40.1827318410232983No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG40.1827318410232983No Hit
ACAGCATCTATTCACAGGGTCTTAGGCTTGGCCTGCCAGAGCCCACGAGA40.1827318410232983No Hit
AGTTAATTCATGTATAAAGCCATAGTGGTCTGTTAGAATTGTGGTTTTCA30.13704888076747374No Hit
CTCATATACCTTCCAATTGATGTTTTTTTGAAACACCCTTAGACAGTGCC30.13704888076747374No Hit
ATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACACTGAT30.13704888076747374No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT30.13704888076747374No Hit
ACAACAGCAGGGCATGTATGAGTAGTTGAGAACGGTGAATAGGAGTATGA30.13704888076747374No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA30.13704888076747374No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA30.13704888076747374No Hit
TATATATTCTAGCTCTGATAAAGTTACAAGATCAATTATATGTACTACAG30.13704888076747374No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG30.13704888076747374No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC30.13704888076747374No Hit
GGCATGTGCGCTGGCAAAGGCTGCAACGAGGCCGACGACGCATTCACGCT30.13704888076747374No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAC100.0066095586145.03
ACAACGC100.0066095586145.01
CAACGCA100.0066095586145.02
AAAAAAA3202.4581168E-67.2565-69
Produced by FastQC (version 0.11.5)