FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_P2_POSCONT_HA_PB2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_P2_POSCONT_HA_PB2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2523231
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGGATGATGATGGGCAT79480.31499295942384986No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG71990.2853087965390406No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT69750.2764312898819014No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC59770.23687882718625444No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG55780.22106576845322526No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA50870.20160659091458533No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG49810.1974056279429034No Hit
CCTATATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGG48650.19280834770974198No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTTT48060.19047007586701337No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT46460.1841289996833425No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA46300.18349489206497543No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA44300.17556854683538686No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT43370.17188279630362815No Hit
ATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGGCTGTT37730.1495305027561884No Hit
ACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGATCATT37610.14905492204241308No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT36030.1427931093110381No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT35220.13958293949305473No Hit
GATGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGT34610.13716540419803022No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG33140.13133954045428262No Hit
TCTAATAACAGTTTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATT31450.12464177873528029No Hit
GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGT31440.12460214700913234No Hit
GATATACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGA30790.12202608480951604No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT30390.12044081576359834No Hit
CATAGTGAATGCACCAAATCATGAGGGAATACAAGCAGGAGTGGATAGAT29730.11782512183783411No Hit
GTATAAGTTTGGTCCCCCATCTGATACCAATAGTCCTGCCCTTGATTGGG29430.11663617005339581No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC29110.11536795481666166No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT28430.11267299743860154No Hit
GGTATATCTTTCGCTCCGAGTCGGCATTTTGGGCCGCCGGGTTATTAGTA28020.11104809666653587No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG27730.10989877660824554No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT27600.10938356416832229No Hit
GATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGA27170.10767939994396074No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC26760.10605449917189508No Hit
GTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG25930.10276506590161583No Hit
TCCTTGAAGAATCCCACCCAGGTATCTTTGAGAACTCATGCCTTGAAACA25270.10014937197585161No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGGT350.003565004662.1522181
TTCGGAC1251.8189894E-1258.000693
TCGGACA1353.6379788E-1253.704344
CGGACAT1405.456968E-1251.786335
ATGTGTA701.698201E-551.7781228
ACTCTGG901.323011E-648.3406141
GACATCT1650.048.3262447
CTTCTAA28550.043.9383131
CATATAC15200.042.9281433
GATGTGT700.0010577141.4224977
TAGATGG30200.041.2853368
TTCTAAT30350.041.0878032
TATACTG35850.040.8512575
TACTGAT16150.040.3965537
AGATGTG907.485162E-540.278266
TGTGTAT907.49216E-540.2718739
ACTGATG16800.039.696568
TTATACT21050.038.91974
GATGTAT13700.038.107931
CCTTATA21550.037.6802642
Produced by FastQC (version 0.11.5)