FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2612154
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTCTTGTTACTACTAATAACCCGGCTCTTGTTACTACTAATAACCCGG28670.10975616292148167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCT5400.034.900367
GTAGCGC5700.034.340436
GCGCTGA5950.031.6742769
TGGGCCG4200.029.343815
AGCGTCA1000.006077672428.9985873
GCAGCGT1050.00770459927.623521
GAGCATC6950.027.1209814
GGATTTA3657.2759576E-1225.82611
CTATACT17250.025.6364334
GATTGGG32050.025.3341792
ACAGCAT14100.025.1938083
AGCGCTG7700.024.4755788
TATACTG22700.023.6332115
ACGTGCA34450.023.3588052
AATATGT1600.00217839422.652111145
GGATTGG37900.022.384891
CGTGCTC86000.022.1704313
TACCCTC8600.021.9146145
TATGTGG8350.021.705536
GTATTAC6700.021.6452941
Produced by FastQC (version 0.11.5)