FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3072586
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC152580.49658496133224583No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT99630.32425455300518846No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT73710.23989564490627763No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC59890.19491724560354046No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA47690.15521127805698523No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA46830.15241233280370348No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT45850.14922283704996378No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT45630.14850682779912427No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG44110.14355985479332392No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT43990.143169304292866No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA42540.1384501524123328No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA42320.1377341431614933No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT39800.12953258265187695No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA37570.12227485251836727No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA37390.12168902676768038No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC34380.1118927183811942No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA32440.10557881862379116No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA32030.10424443774722661No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT31990.10411425424707396No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC30970.10079457499318165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA56350.0124.670444
AGCACGC12800.0124.6223141
GCACGCA13450.0118.0471342
CACGCAC13600.0116.745143
TAACAAG64350.0109.1713945
GAAACAC20600.0109.100844
CGGTGCA4050.0102.0362
AAACACG22950.097.6133965
ACGCACT18150.090.2745744
CGCACTT18150.089.875135
TAGTAAC80100.088.791242
TAGAAAC28350.088.4823462
ACGTGCA14200.082.1998752
AGTAACA86700.082.199293
AACACGT7300.075.478696
ACAAGAG102150.069.115667
GAAGCGG8250.064.158181
AACACGA26700.063.2671476
ACGTCGC350.003569880762.131107145
CGGAGCA8350.061.6460842
Produced by FastQC (version 0.11.5)