FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2751309
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGCCAGCGGAAGACAAGTTTAGTTCCAATGACAAAACAAAGTTCC52780.19183595881087875No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT50250.1826403359273713No Hit
AGTAGAAACAACGCACTTTTTCCCAGTTTATTTGCTGACATTGATTACAA43610.15850636915010272No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC43570.158360983808071No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA41160.14960151695065876No Hit
GGATAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATA39030.14185974748746868No Hit
GTAAGAAGTACAGCAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA37930.13786165058159588No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA37230.13531740709604045No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT35830.13022892012492962No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT33830.12295965302334272No Hit
CTATTATCACATCCTTTTGATTTAATATATGTTATCTAGGTTTAAAACAA33410.12143310693200945No Hit
GCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGG33210.12070618022185077No Hit
CTATTATCCAGGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT33020.1200155998472No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA31930.11605384927683512No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT30260.10998401124701007No Hit
TCATTACATTGTGTCGTCACAAGTCAATTTTGAGTTTTTATATAAATAAG29750.10813034813610539No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT29400.10685822639332769No Hit
GTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATTTATCTAA28960.10525898763097856No Hit
GTAGTAACAAGAGCAGAATTTTAAAACAAAAAACTTATAAAAAGGAAATA27920.10147896873815337No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG27840.10118819805408989No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA13950.0100.828642
AGCACGC14800.096.521511
GCGGTGC9650.090.9238051
TAGTAAC76300.079.534822
GTAGTAA77600.078.774451
GTAACAA78250.076.904224
AGTAACA88750.068.540943
CGCACTT22250.061.9127355
TAACAAG100200.060.2021525
ACGCACG851.3060344E-859.7084164
ACAACGC13200.058.209468
CGAGGAT502.010445E-458.0024575
AACAACG13350.057.5554287
GAAGCGG10300.057.025271
ACGCACT26650.056.8598184
CACGCAC25300.056.4549643
CAACGCA13900.055.278059
ATCGGCA553.2193214E-452.7295075
CGGTGCA10550.052.22972
CGTTTGA1550.051.442577
Produced by FastQC (version 0.11.5)