FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3008805
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT184430.6129676067408822No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC122780.40806898419804544No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT106170.3528643431528464No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA54830.18223181628586765No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG53330.17724644834078646No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA52550.17465405700934425No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC51170.17006751849986954No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT49360.16405184117947158No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA44130.1466695249442885No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA40650.13510347131170014No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT39590.13158047796384278No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT37310.1240027186873194No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG36370.12087855477506851No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA35280.1172558540683095No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA35230.1170896751368068No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA34160.11353344600264889No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC33990.11296843763553968No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG32570.10824895598086283No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGAGGGT31310.10406124690699463No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT30260.1005714893454378No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCATCG100.0070955344144.972877
AGCACGC26200.0133.377271
GCACGCA27050.0129.177552
CACGCAC27300.0127.7290343
GAAACAC15650.0113.953584
CGGTGCA6000.0112.367042
CGCACTT34400.0103.473775
ACGCACT34650.0102.936434
AAACACG17850.099.908895
GTAACAA72300.090.242584
GCGGTGC13450.088.40111
TAACAAG75200.086.5696645
ACGTGCA11250.085.705042
ACAACGC7400.081.302348
CAACGCA7850.078.4884959
GAAGCGG5550.075.7654341
AACACGT5300.075.230526
TAGTAAC95800.068.559862
AACACGA20750.066.730216
CGGTGCC3300.065.904432
Produced by FastQC (version 0.11.5)