FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1904967
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTAGACAGTGCCGTTCAATGTTTTCAAATCTTGTTACTATTACTCTT209301.0987066967564267No Hit
CTCATATACCTTCCAATTGATGTTTTTTTGAAACACCCTTAGACAGTGCC176520.9266302250905134No Hit
GTCTAAGGGTGTTTCAAAAAAACATCAATTGGAAGGTATATGAGAGAAAA172330.9046350934163163No Hit
GTAGTAACAAGAGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGA150670.7909323363606823No Hit
GTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCTCAGCGCGAACA140390.7369681469547766No Hit
GTAGAAGGTGAGGTGATGGAAGCGCCGGCGCGCCAGCGTGAATGCGTCGT102980.5405867923171372No Hit
GTAGAAACACGAGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGA101250.5315052701700345No Hit
CTTTAATATATTGGGGTTTCATGAGAGTCTTAGTGTCATAAAAAGTTCTG85850.45066397475651804No Hit
GTCTTAGTGTCATAAAAAGTTCTGCTGCTAAAAGAGTAATAGTAACAAGA81870.42977122438341453No Hit
GAAGCGGAGCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCGGCGCG80370.42189707223274736No Hit
TCGTAGAACACCACGCGCTTGCTGGCGCCGAAGCCGGTGAAGTACGCATT76380.40095182751197267No Hit
ACTTAGAGTCACCACTTCATTTCTCTGGGCTCTAGTTAATTCATGTATAA72890.3826313001747537No Hit
ATATTAAAGAGGTAAAATCAGGGATAGAGGATTCATAGCCATACCTGATC72650.3813714358306469No Hit
GCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCGGCGCGCCAGCGTG63690.3343365003173283No Hit
GGCAAAGGCTGCAACGAGGCCGACGACGCATTCACGCTGGCGCGCCGGCG63450.3330766359732216No Hit
GAAGCGGAGCGGGCACTTGAAGCAAATTTTACGGGGGGTGTTCATATGGT61750.3241525968691321No Hit
GTTCTACGACACGCTGCTGCGGCAGCTCAGCGCCGGCGAGGTCGAGGCCG57400.3013175556321973No Hit
GTAGAAACACGTGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGA56560.2969080304278237No Hit
GCCGTAGAAGGTGAGGTGATGGAAGCGCCGGCGCGCCAGCGTGAATGCGT54730.28730156480400976No Hit
GCCTGTAACAATATAATAAAAGAGGCAGACATGTTGAAGACAGATTTTCC53290.27974237873936925No Hit
GGCCCAGCTCGTGCGCCAGCACGGCCTCGACCTCGCCGGCGCTGAGCTGC53070.2785875030906047No Hit
TGCCAATGCGTACTTCACCGGCTTCGGCGCCAGCAAGCGCGTGGTGTTCT52100.2734955513665066No Hit
GCTAAAAGAGTAATAGTAACAAGATTTGAAAACATTGAACGGCACTGTCT51800.27192072093637315No Hit
GGTGATGGAAGCGCCGGCGCGCCAGCGTGAATGCGTCGTCGGCCTCGTTG51620.2709758226782931No Hit
ATCCTTAACAAAGCCAAGAAAACCCACAGCCTGTAACAATATAATAAAAG51490.2702933961585686No Hit
AGTAGAAACAACGCACTTGGCCAAGCTTGTCTGTCTGTTCGTTCTTTCTT51450.27008341876788416No Hit
GTTAGAATTGTGGTTTTCAAACTGCTCTTCAGAGCTCTGAGAAGGTAGTT51350.26955847529117305No Hit
CAGCCGAGCAGCGCGAAGCCCGCGAGGCTCAGCGCGAACAGCGTGACCAT51000.267721173122684No Hit
ATTTCAAGCACCGCCACATCGTCAAGCGCATGGTCACGCTGTTCGCGCTG50170.2633641422659815No Hit
GTATAAAGCCATAGTGGTCTGTTAGAATTGTGGTTTTCAAACTGCTCTTC49640.26058194183941247No Hit
AGCTGGGCCATTTCAAGCACCGCCACATCGTCAAGCGCATGGTCACGCTG49070.2575897640221589No Hit
CTATTACCGCTTCTCGTTGCGCTTGGATTCACTGTTGCAGAAGTAGAATC48490.2545450918572343No Hit
AAACACGAGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCT47650.25013556665286063No Hit
GTATATGAGAGAAAAGAAGACCTTGAAAATGGGGAAAATCTGTCTTCAAC47510.24940064578546506No Hit
GCTTGAAATGGCCCAGCTCGTGCGCCAGCACGGCCTCGACCTCGCCGGCG47410.24887570230875392No Hit
CTGCTGCGGCAGCTCAGCGCCGGCGAGGTCGAGGCCGTGCTGGCGCACGA47060.24703840014026487No Hit
GCGCTTGACGATGTGGCGGTGCTTGAAATGGCCCAGCTCGTGCGCCAGCA45980.24136901059178453No Hit
ATATTGGGGTTTCATGAGAGTCTTAGTGTCATAAAAAGTTCTGCTGCTAA45180.23716946277809536No Hit
GCGGAGCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCGGCGCGCCA44170.2318675336633128No Hit
GTAGAACACCACGCGCTTGCTGGCGCCGAAGCCGGTGAAGTACGCATTGG43090.2261981441148324No Hit
AGTTAATTCATGTATAAAGCCATAGTGGTCTGTTAGAATTGTGGTTTTCA42640.22383589846963228No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA42180.221421158476761No Hit
GAGCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCGGCGCGCCAGCG42020.2205812489140232No Hit
GTGTTCTACGACACGCTGCTGCGGCAGCTCAGCGCCGGCGAGGTCGAGGC40810.21422943284581833No Hit
AGGTGAGGTGATGGAAGCGCCGGCGCGCCAGCGTGAATGCGTCGTCGGCC40310.21160471546226262No Hit
GTTGCAGCCTTTGCCAGCGCACATGCCAATGCGTACTTCACCGGCTTCGG40280.2114472324192493No Hit
GGTGAGGTGATGGAAGCGCCGGCGCGCCAGCGTGAATGCGTCGTCGGCCT39880.20934745851240466No Hit
AAGGTGAGGTGATGGAAGCGCCGGCGCGCCAGCGTGAATGCGTCGTCGGC39790.20887500938336465No Hit
GTTGAAGACAGATTTTCCCCATTTTCAAGGTCTTCTTTTCTCTCATATAC39690.2083500659066535No Hit
GACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCTCAGCGCGAACAGCGT39320.20640777504282226No Hit
TTTTCAAGGTCTTCTTTTCTCTCATATACCTTCCAATTGATGTTTTTTTG38940.2044129898313199No Hit
GCGTGAATGCGTCGTCGGCCTCGTTGCAGCCTTTGCCAGCGCACATGCCA38720.20325811418255538No Hit
CACATACCCTTTTCCAACTACCTTCTCAGAGCTCTGAAGAGCAGTTTGAA37990.19942602680256402No Hit
CTTCACCGGCTTCGGCGCCAGCAAGCGCGTGGTGTTCTACGACACGCTGC37760.1982186568061284No Hit
GCAGAAGCGGAGCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCGGC37040.19443906377380815No Hit
GAGCAGAAGCGGAGCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCG36990.1941765920354526No Hit
AAACAACAGCATCCTTAACAAAGCCAAGAAAACCCACAGCCTGTAACAAT36800.1931791994297014No Hit
GTAGTAACAAGAGCAAGAACTATTACCGCTTCTCGTTGCGCTTGGATTCA36620.19223430117162135No Hit
CACTAAGACTCTCATGAAACCCCAATATATTAAAGAGGTAAAATCAGGGA35970.1888221685729989No Hit
GCCTCGTTGCAGCCTTTGCCAGCGCACATGCCAATGCGTACTTCACCGGC35650.18714234944752323No Hit
TGCTGGCGCCGAAGCCGGTGAAGTACGCATTGGCATGTGCGCTGGCAAAG35160.1845701264116386No Hit
GGCATGTGCGCTGGCAAAGGCTGCAACGAGGCCGACGACGCATTCACGCT34940.1834152507628741No Hit
CGTTGCAGCCTTTGCCAGCGCACATGCCAATGCGTACTTCACCGGCTTCG34740.1823653638094518No Hit
TAACAAGAGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCT34530.18126298250835843No Hit
GCCTTTGCCAGCGCACATGCCAATGCGTACTTCACCGGCTTCGGCGCCAG34190.17947817468754051No Hit
GCCTCGACCTCGCCGGCGCTGAGCTGCCGCAGCAGCGTGTCGTAGAACAC34050.17874325382014492No Hit
GAGTAATAGTAACAAGATTTGAAAACATTGAACGGCACTGTCTAAGGGTG33740.17711592904234036No Hit
CGCCAGCACGGCCTCGACCTCGCCGGCGCTGAGCTGCCGCAGCAGCGTGT33380.17522613252618024No Hit
GGTCGAGGCCGTGCTGGCGCACGAGCTGGGCCATTTCAAGCACCGCCACA33320.17491116644015356No Hit
ATGTGGCGGTGCTTGAAATGGCCCAGCTCGTGCGCCAGCACGGCCTCGAC33280.1747011890494691No Hit
GCGTACTTCACCGGCTTCGGCGCCAGCAAGCGCGTGGTGTTCTACGACAC32890.17265390949029563No Hit
CAGAAGCGGAGCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCGGCG32870.1725489207949534No Hit
GGCCAAGCTTGTCTGTCTGTTCGTTCTTTCTTTCTTTCTTTCTTTCTTTC31940.16766694646153976No Hit
GCGCGAAGCCCGCGAGGCTCAGCGCGAACAGCGTGACCATGCGCTTGACG31890.1674044747231842No Hit
CCCAGCCGAGCAGCGCGAAGCCCGCGAGGCTCAGCGCGAACAGCGTGACC31860.16724699168017082No Hit
GAATTAACTAGAGCCCAGAGAAATGAAGTGGTGACTCTAAGTTGCTTGAA31830.1670895086371575No Hit
CTATTACTCTTTTAGCAGCAGAACTTTTTATGACACTAAGACTCTCATGA31430.16498973473031292No Hit
GATGGAAGCGCCGGCGCGCCAGCGTGAATGCGTCGTCGGCCTCGTTGCAG31260.16409733081990396No Hit
CTACGACACGCTGCTGCGGCAGCTCAGCGCCGGCGAGGTCGAGGCCGTGC30960.16252250038977054No Hit
GAAGTACGCATTGGCATGTGCGCTGGCAAAGGCTGCAACGAGGCCGACGA30330.15921535648649032No Hit
TCACCGGCTTCGGCGCCAGCAAGCGCGTGGTGTTCTACGACACGCTGCTG29890.15690560518896127No Hit
TCTCAGAGCTCTGAAGAGCAGTTTGAAAACCACAATTCTAACAGACCACT29650.15564574084485455No Hit
CACGAGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCTCAG29610.15543576345417007No Hit
GAAGCAGAGCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCGGCGCG29410.15438587650074778No Hit
AACTATTACCGCTTCTCGTTGCGCTTGGATTCACTGTTGCAGAAGTAGAA29240.15349347259033883No Hit
GCCGAGCAGCGCGAAGCCCGCGAGGCTCAGCGCGAACAGCGTGACCATGC28850.15144619303116535No Hit
CCTTCCAATTGATGTTTTTTTGAAACACCCTTAGACAGTGCCGTTCAATG28430.14924143042897856No Hit
AACAAGAGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCTC28190.1479815660848718No Hit
TTAATATATTGGGGTTTCATGAGAGTCTTAGTGTCATAAAAAGTTCTGCT28160.14782408304185846No Hit
GCCATACCTGATCTCACATACCCTTTTCCAACTACCTTCTCAGAGCTCTG28070.14735163391281844No Hit
GTCATAAAAAGTTCTGCTGCTAAAAGAGTAATAGTAACAAGATTTGAAAA27640.14509437696296051No Hit
TAATAAAAGAGGCAGACATGTTGAAGACAGATTTTCCCCATTTTCAAGGT27130.14241716523173367No Hit
GTGTTCATATGGTCAATTTTGGGGGAAGGCATAGGCTAAATTTTTTCTTC27020.14183972740735143No Hit
AAGCAGAGCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCGGCGCGC26670.14000242523886242No Hit
ATTAGTAGAAACACGAGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCC26560.13942498741448014No Hit
AACACGAGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCTC26480.13900503263311123No Hit
AGCGTGAATGCGTCGTCGGCCTCGTTGCAGCCTTTGCCAGCGCACATGCC26360.13837510046105786No Hit
GAAGGTGAGGTGATGGAAGCGCCGGCGCGCCAGCGTGAATGCGTCGTCGG26240.1377451682890045No Hit
GGCTGCAACGAGGCCGACGACGCATTCACGCTGGCGCGCCGGCGCTTCCA26110.13706274176928No Hit
ACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCTCAGCGCGAACAGCGTGAC25960.13627532655421326No Hit
ACCATGCGCTTGACGATGTGGCGGTGCTTGAAATGGCCCAGCTCGTGCGC25580.13428054134271092No Hit
CGCTTGCTGGCGCCGAAGCCGGTGAAGTACGCATTGGCATGTGCGCTGGC25540.13407056395202646No Hit
AGCAGAAGCGGAGCATGAAGCCGTAGAAGGTGAGGTGATGGAAGCGCCGG25540.13407056395202646No Hit
AGTAGAAACAACGCACTCGGCCAAGCTTGTCTGTCTGTTCGTTCTTTCTT25360.1331256656939464No Hit
TCTCTGGGCTCTAGTTAATTCATGTATAAAGCCATAGTGGTCTGTTAGAA25310.13286319395559085No Hit
AAGCCATAGTGGTCTGTTAGAATTGTGGTTTTCAAACTGCTCTTCAGAGC25100.13176081265449743No Hit
GGGATAGAGGATTCATAGCCATACCTGATCTCACATACCCTTTTCCAACT24960.13102589178710183No Hit
GTCTAAGGGTGTTTCAAAAAAACATCAATTGGAAGGTATATGAGCTGTCT24850.13044845396271956No Hit
TTGCAGCCTTTGCCAGCGCACATGCCAATGCGTACTTCACCGGCTTCGGC24330.12771874788382162No Hit
CACGTGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCTCAG24020.12609142310601706No Hit
GACATGTTGAAGACAGATTTTCCCCATTTTCAAGGTCTTCTTTTCTCTCA23900.1254614909339637No Hit
GTAACAAGAGCGGTCGGACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGC23540.12357169441780357No Hit
GCCATAGTGGTCTGTTAGAATTGTGGTTTTCAAACTGCTCTTCAGAGCTC23520.12346670572246134No Hit
ACACCCAGCCGAGCAGCGCGAAGCCCGCGAGGCTCAGCGCGAACAGCGTG23390.12278427920273684No Hit
CTCATATACCTTCCAATTGATGTTTTTTTGAAACACCCTTAGACCTGTCT23150.1215244148586301No Hit
GTGCTGGCGCACGAGCTGGGCCATTTCAAGCACCGCCACATCGTCAAGCG23000.1207369996435634No Hit
GCTCTTCAGAGCTCTGAGAAGGTAGTTGGAAAAGGGTATGTGAGATCAGG22930.12036953920986557No Hit
GAACACCACGCGCTTGCTGGCGCCGAAGCCGGTGAAGTACGCATTGGCAT22720.11926715790877218No Hit
CACTTGGCCAAGCTTGTCTGTCTGTTCGTTCTTTCTTTCTTTCTTTCTTT22200.11653745182987421No Hit
CAGCGCGAACAGCGTGACCATGCGCTTGACGATGTGGCGGTGCTTGAAAT22050.11575003661480751No Hit
CAGCGTGACCATGCGCTTGACGATGTGGCGGTGCTTGAAATGGCCCAGCT21930.11512010444275413No Hit
GCGTGACCATGCGCTTGACGATGTGGCGGTGCTTGAAATGGCCCAGCTCG21800.11443767792302963No Hit
TCATGAGAGTCTTAGTGTCATAAAAAGTTCTGCTGCTAAAAGAGTAATAG21510.11291534184056731No Hit
GTAACAAGATTTGAAAACATTGAACGGCACTGTCTAAGGGTGTTTCAAAA21390.11228540966851394No Hit
AGCGCGAACAGCGTGACCATGCGCTTGACGATGTGGCGGTGCTTGAAATG21270.11165547749646056No Hit
CTTCAGAGCTCTGAGAAGGTAGTTGGAAAAGGGTATGTGAGATCAGGTAT21110.11081556793372273No Hit
TTCATAGCCATACCTGATCTCACATACCCTTTTCCAACTACCTTCTCAGA20730.10882078272222039No Hit
GAGCTGCCGCAGCAGCGTGTCGTAGAACACCACGCGCTTGCTGGCGCCGA20150.10577611055729574No Hit
GGCATAGGCTAAATTTTTTCTTCGACAAGCGGGGCCGATTCTACTTCTGC20120.10561862751428241No Hit
AGTAGAATCGGCCCCGCTTGTCGAAGAAAAAATTTAGCCTATGCCTTCCC19940.10467372925620233No Hit
GTACTTCACCGGCTTCGGCGCCAGCAAGCGCGTGGTGTTCTACGACACGC19480.10225898926333107No Hit
TACCTGATCTCACATACCCTTTTCCAACTACCTTCTCAGAGCTCTGAAGA19410.10189152882963327No Hit
GGTATGGCTATGAATCCTCTATCCCTGATTTTACCTCTTTAATATATTGG19270.10115660796223766No Hit
TAGTAACAAGATTTGAAAACATTGAACGGCACTGTCTAAGGGTGTTTCAA19200.10078914752853986No Hit
GAGCGGGCACTTGAAGCAAATTTTACGGGGGGTGTTCATATGGTCAATTT19170.10063166448552653No Hit
CTGTTAGAATTGTGGTTTTCAAACTGCTCTTCAGAGCTCTGAGAAGGTAG19070.10010672100881537No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGGA100.0070952238144.9736145
GTAGTAA34500.099.42221
CGTCAGA406.118589E-790.6275252
GCGTCAG451.2315722E-680.574721
CGGAGCG15600.079.9381265
GAGCAGA17050.079.535051
GGAGCGG14350.078.312296
GCGGGGG502.30954E-672.486799
AAGAGCG35900.064.8140959
AACAACG21800.062.1790437
GGAGCGT350.003566604362.1445926
AGCGGGC22950.061.905948
ACAACGC21950.061.7541288
GAGCGAT1700.059.6950077
CAACGCA22750.059.263939
AAGCGGA44900.058.615222
CGGTGCA6100.058.2393262
GGTAGTA502.0083447E-458.01381
TAGTAGT9100.055.7707863
GGTAGAG400.00604602954.3879431
Produced by FastQC (version 0.11.5)