FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3017771
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT125110.41457751433094164No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC112540.3729242543585978No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT98870.327625919925667No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA60370.20004831380512306No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC49410.16373011736145654No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG48540.1608471948335377No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT44840.1485864898297452No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC37370.12383312053830459No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA36070.11952530526670181No Hit
AAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACA33960.11253338971048499No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG30990.10269168866690018No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG30270.10030582174724324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC23350.0123.578691
GCACGCA24800.0116.3456042
CACGCAC26850.0108.272623
CGCACTT30000.0102.2204365
ACGCACT31150.099.377584
CGGTGCA8100.097.5573652
TAACAAG85300.095.699135
AAACACG23850.092.10285
GAAACAC24000.091.829234
GCGGTGC16150.082.153471
GTAACAA99650.081.772564
TAGTAAC106250.077.375372
GTAGTAA108400.076.78211
AACACGT8300.075.990586
ACGTGCA15450.073.6698462
AGTAACA114600.071.674373
CCGATTG651.4012039E-766.907899
ACGAGCG651.4012039E-766.907899
ACAAGAG125600.065.5006567
GTCTCGC9450.061.376991
Produced by FastQC (version 0.11.5)