FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1831612
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC84460.4611238624774243No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT74850.4086564184991145No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT51840.283029375217022No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC50810.27740591347949234No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT48640.26555842612955144No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT36220.19774930498380663No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT35250.19245342354166714No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA31040.16946820614846375No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA27430.14975879170916112No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC27210.1485576639593975No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG26760.146100811743972No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA24500.13376195395094595No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT24450.1334889703714542No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT24290.13261542291708067No Hit
CAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTT23880.13037695756524853No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC21810.11907543737429106No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC21790.11896624394249436No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA21500.11738293918144235No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA20700.11301520190957473No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGT20170.11012157596696243No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC20140.1099577858192674No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC19980.10908423836489387No Hit
TAATATATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACC19940.10886585150130049No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT19460.10624520913817992No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC19280.10526246825200972No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC19030.10389755035455107No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC18920.10329698647966927No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAACA18620.10165908500271892No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT18520.10111311784373547No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA18520.10111311784373547No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTG100.007093708144.983816
GTAACAA43800.0128.267644
CGGTGCA4350.0123.319572
CACGCAC5100.0122.241253
AGCACGC5150.0119.6664351
GCACGCA5200.0118.4963762
TAACAAG49150.0114.305655
GAAACAC14100.0113.6220554
AAACACG14850.0107.8835755
ACAGCGT301.6007032E-596.6558764
TAGTAAC59750.094.633782
TAGAAAC17950.094.098132
CTTGGTC406.1285573E-790.602519
CGCACTT7150.089.220815
ACGCACT7250.088.990064
AGTAACA64600.087.641143
ACGTGCA8600.085.978772
CAGCCTT2550.085.284595
AACACGT4550.084.4411246
GAGCAGA2500.084.1043851
Produced by FastQC (version 0.11.5)