FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2992834
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT93160.3112768700168469No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG64810.2165506005344767No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTA52360.17495123351311834No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC50760.16960513012081524No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA46220.15443556174515527No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT43700.1460154489022779No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT38180.1275713921988323No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT36010.12032073947302123No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA35810.11965247654898334No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTA34870.11651164080600529No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG34560.11547583327374655No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA34540.11540900698134277No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT34240.11440661259528595No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG33360.11146625572951924No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC33180.11086481909788515No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT31590.10555212885178396No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA30370.10147572501515287No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT30040.10037309119049036No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGGA1900.057.2388234
CGAGGAT2450.050.307865
CGGTGCA16050.046.979822
CACGAGG4600.044.131963
ATCACGA2150.043.8467861
CCGTTCA907.4940865E-540.270429
ACGAGGT2450.038.470724
CGTCCAG2109.094947E-1237.97755
CAAGAGG79450.036.7679868
ACGTGCA15350.036.3693352
GGCTATC2950.034.4079673
TCACGAG2750.034.2739142
CGAGCGG2353.45608E-1133.9373445
TTGGAAT48350.033.732204145
GCACGCA76200.033.3016742
CTGAACG1102.4399186E-432.948539
CACGCAC78100.032.398693
GCACTTT63100.030.908366
GTCTCGC1703.0092324E-629.8594651
ACTTTCT65100.029.0615128
Produced by FastQC (version 0.11.5)