FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684824
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT38630.22928210899179977No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC33940.20144537352269434No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC29530.17527053270846094No Hit
GTAGTAACAAGAGGATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT24160.14339776736323795No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT23110.13716566240746808No Hit
GAAACATTGTCTCTGGAGACCATATAAACAACAAAGATAGCTATCATCAG20380.12096218952246646No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA18930.11235594934545091No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT18710.1110501749737658No Hit
GTCTTAAAATACTGGTTAATTCCGCTACCAAAACGTCTTGGGAATCCTGC17710.10511483692065166No Hit
AAGCAGAGCAGAGGAGGAGAGGGTTCCCAGGGCTGTGTGTGTTAAGTGGG17360.1030374686020617No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAAC203.869003E-4108.732468
GTAACAA50550.098.538664
TAGTAAC55850.089.836752
TAACAAG55800.089.397495
CGGTGCA4450.087.984232
CGCACTA259.3850336E-487.0066155
ACAAGAG65450.076.309317
AGTAACA66250.075.8435063
CGCACTG851.6916601E-1068.240495
CGTGCAC2750.065.914113
CGTCAGA554.060974E-665.914112
AAACACG17850.063.3661735
TGCGGCG350.003565851862.1475875
AAGAGCG10500.060.7521069
CGGAGCG1200.060.421275
GTAGTAA84800.059.77641
CAAGAGC55650.059.6579448
ACGCACT4500.059.6156464
GCACGCA3800.059.1492462
AACAAGA85600.058.444876
Produced by FastQC (version 0.11.5)