FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2592111
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCCT123720.47729437512513934No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT119000.4590852783696377No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA112570.4342792418997489No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT102700.39620216881144366No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA92260.35592611581834266No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC76600.29551203632869116No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCC67300.2596339431451817No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT64650.2494106155176225No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC63930.2466329566905121No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA58760.22668782316806652No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG57770.22286854228078964No Hit
ATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG52820.20377213784440557No Hit
GTAGAAACAACAGCAACAAAAAAGAAAGGCCTGATTCTGGCTGAGAGAAA52260.20161173653443082No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT51190.1974838268885862No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC49960.1927386597256059No Hit
GTCTATTGGAATTGAAGGGTTTGAGCCATACTGTATGAACAGTTCCTCAA48680.18780059958852072No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA48500.1871061848817431No Hit
CATAATAATTGAGGGGCTTTCTGCCGAAGAGATAATAAAAATGGGTGAAA45460.1753782920561658No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG45390.17510824189241894No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT45390.17510824189241894No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCAGGCACTGAAG44850.17302499777208613No Hit
TTCAATACCTCCATGTTGTCAGAGAGTACTTCCCTCATTGTTTCTTTGGC41910.16168289089471863No Hit
GGATGTAATGGAAGTGCTAAAGCAGAGCTCCATGGGAAATTCAGCTCTTG40290.1554331585337202No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA40270.15535600134407823No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC39100.1508423057500238No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC37860.14605855999222256No Hit
TAATAATTGAGGGGCTTTCTGCCGAAGAGATAATAAAAATGGGTGAAACA37850.14601998139740158No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG37730.14555703825954985No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG37530.14478546636313028No Hit
ACATTGGAGTGCTGAGATCTCTTGGGGCAAGTCAAAAGAATGGGGAAGGG37510.14470830917348831No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT36920.14243217207905062No Hit
CTCCAATGTTGCTTTGCAACTCTTCTGCCAGCTTTTGGACGTCTTCTCCT36590.14115907844995834No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT36520.14088902828621153No Hit
ATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGAGA35580.13726264037303956No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA35090.13537228922681166No Hit
GATTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAATTGAATTT35070.1352951320371697No Hit
CCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGA34460.13294183775309004No Hit
CTTTTGACTTGCCCCAAGAGATCTCAGCACTCCAATGTTGCTTTGCAACT33680.12993270735705378No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG32970.1271936271247643No Hit
GTACTTCCCTCATTGTTTCTTTGGCTTGGATTTCTTTTTGGTAACTGTGT32750.12634489803870283No Hit
GCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAAT32700.12615200506459792No Hit
TAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAATGCAGA32520.12545759035782034No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC32050.12364439640123435No Hit
GTAGTAACAAGAGCAACAAAAAAGAAAGGCCTGATTCTGGCTGAGAGAAA31920.12314287466856165No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA31680.12221698839285818No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT31540.12167688806536449No Hit
GATTCTGGCTGAGAGAAAAATGAGAAGATGTGTGAGCTTTCATGAAGCAT31380.12105963054822882No Hit
GGACAATAGGGCATTTGAATCAAATAAAAAGAGGAATAAACATGAAAATA31350.12094389476376591No Hit
CTCTTGGGGCAAGTCAAAAGAATGGGGAAGGGATTGCAAAGGATGTAATG31160.12021090146216733No Hit
ATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCCTCAT30210.11654593495417441No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATTGTAAG29490.113768276127064No Hit
GAATAAAGACTCATAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT29210.11268807547207661No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG29170.11253376109279271No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT29080.11218655373940391No Hit
TTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGA29050.11207081795494098No Hit
CCAACAGACGGATTCATAAATAGGAGTACTTTCATCATTTTTACTCCAAT28940.11164645341191022No Hit
CTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATCTTA28630.11045051697245989No Hit
TTCATGAAGCATTTGAAATAGCAGAAGGCCATGAAAGCTCAGCGCTACTA28560.11018046680871306No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATT28400.10956320929157741No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG28400.10956320929157741No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA28310.1092160019381886No Hit
AAACAACAGCAACAAAAAAGAAAGGCCTGATTCTGGCTGAGAGAAAAATG27900.1076342795505285No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT27860.1074799651712446No Hit
TGATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT27830.10736422938678167No Hit
AAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAACT27690.10682412905928798No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA27240.10508809229234396No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC26880.10369926287878875No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC26720.10308200536165311No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGAT25950.10011145356043781No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTC3350.097.385453
CGGTGCA5050.096.185552
GTAACAA39750.093.381164
GTCTCGC3600.092.647131
TAACAAG42900.086.017525
CGCCTAG451.2336386E-680.5534065
AGTAACA46900.079.763323
TAGTAAC47400.078.310142
CAACGCA26150.070.686469
AGCACGC2100.069.0537641
ACAACGC27500.067.21648
GTAGTAA58300.065.168751
TCGCTCT5050.064.6022344
AACAACG30050.061.994977
GGTGCAT7100.058.202673
TCGCCTA651.09430675E-555.7677354
CTGTCGC3250.055.760219
CAGCGCA400.00605260954.3735475
CGGAGCA6700.053.020972
AACAAGA70850.052.186326
Produced by FastQC (version 0.11.5)