FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2533691
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAAGAAGGCTGGAAGGTATTTGGCAGGGGGGCGACAGAAATAGAA104490.41240230162241565No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC62850.2480570835196557No Hit
GTAGTAACAAGAGCTTGCTTAAAAGATACAATACAGAATTCTTAACAGAG58780.23199356196158097No Hit
CTTCTACACCATATGAATTAAAAGCACATCAGTGAGAAAATTAACTGGAA51100.20168205199450132No Hit
AACAACAGCTGTGTAGGGTAGCTAGCAGTTAGTGTTGTACAGAAGACAGA42510.1677789438412182No Hit
ACTTCTACACCATATGAATTAAAAGCACATCAGTGAGAAAATTAACTGGA40580.160161598237512No Hit
GTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATTTATCTAA40020.15795138396907912No Hit
ATGTAATGATGGCATTGAGTCTTTGAGAACAGTTTTTTACTCCTTTATAC39320.155188616133538No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG38850.1533336148725318No Hit
CTTATACATAATGGATATGAAAGCACAGAAGAGGTGGAGCCAGGACAACT38480.15187329473088865No Hit
GTGTAGGGTAGCTAGCAGTTAGTGTTGTACAGAAGACAGATATTTGTGCA38420.15163648605927085No Hit
GTAGTAACAAGAGCAATCACTTCTCATTTCTCACTGTTTCTGTGCATAAG37610.1484395689924304No Hit
AAATAAGGCAACAATGAGAAACGTTACCTACAAGATGGCAGAAGACCAGA35890.14165105373938652No Hit
GTGTAGAAGTAGGCTGGAAGGTATTTGGCAGGGGGGCGACAGAAATAGAA35490.14007232926193447No Hit
CTTTTACACCATATGAATTAAAAGCACATCAGTGAGAAAATTAACTGGAA34890.13770424254575636No Hit
ATTATATGTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATT34020.13427051680729812No Hit
GTTAAAACTATGTTCAAGTGATTATGTCAGTCATTGTAGGAAGAAAAATA33550.13241551554629194No Hit
TCATTACATTGTGTCGTCACAAGTCAATTTTGAGTTTTTATATAAATAAG33380.1317445576433748No Hit
GAAGTAGGCTGGAAGGTATTTGGCAGGGGGGCGACAGAAATAGAACCATG31900.12590327707680218No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT31780.12542965973356657No Hit
TCTATGTACCAAGCACTCTGCCAAGTGCTCAGTGTGACTTAATTGAATCT31660.12495604239033094No Hit
GTATAGAATTGCTGGGCTGCAAAGATTCAATTAAGTCACACTGAGCACTT31330.12365359469643299No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA31200.12314050924126106No Hit
TATATATTCTAGCTCTGATAAAGTTACAAGATCAATTATATGTACTACAG30300.11958837916699393No Hit
TTACTAAACTGTGTGGAAGGCTTCCAGAGATTTAATAGTCTACCTCGAGG30120.11887795315214049No Hit
ACAACAGCTGTGTAGGGTAGCTAGCAGTTAGTGTTGTACAGAAGACAGAT29850.11781231412986036No Hit
GTAGTAACAAGAGCGAAACTCCATCAAAAAGAAAAAAAAAGAAAGAGAGA28620.11295773636169525No Hit
ATTCTATACATTGGGTTATTGTCTCCATTTCACATATCAGCACACTGAGG27450.10833996726514795No Hit
TTCTAGCTCTGATAAAGTTACAAGATCAATTATATGTACTACAGATTGCT26950.10636656166833289No Hit
CAACAGCTGTGTAGGGTAGCTAGCAGTTAGTGTTGTACAGAAGACAGATA26330.10391953872828219No Hit
GCCTTCTTTTACACCATATGAATTAAAAGCACATCAGTGAGAAAATTAAC25860.10206453746727599No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA64500.098.69351
GTAACAA69500.090.4421544
CGGTGCG259.388543E-486.999722
TAACAAG78950.079.800255
TAGTAAC79350.079.763442
AGTAACA83050.076.122573
AAGAGCG28150.063.6018079
CAAGAGC104800.059.7590038
AACAAGA106850.059.0311626
CGAGCGA502.0108678E-457.9998135
CGGTGAG20750.057.988365145
GCACGCA5800.057.499812
ACAAGAG110200.057.4884647
CGTGCAA2400.057.395653
AACACGA20450.056.371231
CTACGGG400.006052030354.3748253
TCGTCGG400.006056757654.364094145
GTAAAAG31050.053.9370233
AGCACGC6700.050.860051
ACACGAG23100.050.530142
Produced by FastQC (version 0.11.5)