FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2778008
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT105800.3808484352816839No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC92080.3314605285513937No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC85580.30806246778267016No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT75470.2716694840331633No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT70070.2522310950868392No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG65390.23538449133335826No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA63510.2286170522187121No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA60440.21756596813256118No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT57400.20662287509611205No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG50060.18020106493573812No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT46460.16724213897152204No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA45240.16285050295031547No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC45220.16277850891718093No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA44820.16133862825449027No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA43800.15766693256462905No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA42900.15442720107357502No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC42390.15259135322864442No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT41040.1477317559920634No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG39960.14384407820279857No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGT39350.14164826019219529No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT38660.13916446604905386No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA37990.13675266593904697No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA37370.1345208509118764No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT37350.1344488568787419No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG37120.13362092549769475No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG37030.13329695234858935No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA35580.12807738494633564No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA35090.12631353113453958No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC34640.12469366538901255No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCCT34450.12400972207423448No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT33790.12163391898079487No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT33520.12066199953347867No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC33390.12019403831810418No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG32560.11720628594302103No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAACTCACTC32520.11706229787675199No Hit
ATATAAATCCTTATTTTCTCTTCATAGATGTGCCCGTACAGGCAGCAATT32070.11544243213122496No Hit
TGATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT31890.11479448583301417No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC31640.1138945604188325No Hit
GAATAAAGACTCATAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT30960.11144676329225833No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC30820.11094280506031659No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT30510.10982689754673132No Hit
TTTGTGTATCGCATGTATTCAATGATGACCAATAACCCCATAAACATCTT30510.10982689754673132No Hit
GTTCATACAGTATGGCTCAAACCCTTCAATTCCAACAGACGGATTCATAA30390.10939493334792413No Hit
CAACAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG30130.10845901091717518No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG29970.1078830586520989No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG29840.10741509743672445No Hit
GTAGTAACAAGAGGATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT29840.10741509743672445No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG29830.10737910042015718No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT29560.10640718097284098No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT29430.10593921975746651No Hit
AACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTATTCAAT29380.10575923467463016No Hit
GGATTTAATGGAAGTGCTAAAGCAGAGCTCCATGGGAAATTCAGCTCTTG29380.10575923467463016No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA29340.10561524660836111No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGAT29260.10532727047582296No Hit
GCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGTG29020.10446334207820855No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA28940.10417536594567042No Hit
GCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGTG28730.10341942859775782No Hit
GTTTCTAATAATGATAATGATACAACCATATCAGCTATTATGGAGCTGTT28530.10269948826641248No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC28440.10237551511730707No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA28390.10219553003447074No Hit
ACACAATAGACACCGTGATCAGAACGCATGAGTACTCAAACAAGGGGAAA28310.10190755390193261No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA27980.1007196523552128No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG301.1009979E-7120.8558961
AGCGAAG301.1019438E-7120.838492
CGTCAGA352.7623537E-7103.575862
GCGAAGC406.1187893E-790.6288763
GTAACAA99250.088.172544
TAACAAG103450.084.662875
AAAGCGG554.3959517E-879.094295
TAGTAAC116500.075.552582
AGTAACA121100.072.742583
TACGGAG1500.072.50313
CTACGGA1500.072.50312
GAGCGGT406.66386E-572.50314
GCTACGG1550.070.174391
CAGCCTT4850.068.765825
ACAAGAG132350.064.9018557
GATTCCG350.003569620462.1320887
AACAAGA140450.061.2753146
CAAAGCG606.807046E-660.4192474
CGGTGCA6600.059.3207172
GAGCGCA502.0104452E-458.0024764
Produced by FastQC (version 0.11.5)