FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2769569
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA89500.3231549746549012No Hit
CACGAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG63430.22902480494257407No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG62020.22393376009046895No Hit
CACGTGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG59360.2143293776035188No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT51620.18638279096855864No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG50230.181363959518611No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT47240.1705680558960618No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA43050.1554393481440614No Hit
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC40770.14720702029810415No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAATCTCAGTAAGAACAATT40430.1459793924614263No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC38400.13864973214243803No Hit
ACACGAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATA36540.13193388574178871No Hit
ACACGTGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATA36080.13027297749216576No Hit
ATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAAT34710.12532635944437565No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC33480.12088523521168817No Hit
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG33270.12012699448903422No Hit
ACAACAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAATCCTGAG32060.11575808365850426No Hit
AACACGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACC31670.11434992231643262No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT31040.11207520014847076No Hit
AACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACC27810.1004127357000313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA93300.0109.7954254
TAGTAAC94650.0108.152812
GTAGTAA95750.0107.067551
GCACGCA30300.0105.994862
AGCACGC30450.0105.240331
AGTAACA104150.098.426923
TAACAAG105350.097.4434055
CGCACTT34900.096.386515
CACGCAC34750.093.04733
ACGCACT37550.090.356554
AAACACG39350.079.406545
ACGTGCA26800.076.8258362
ACAAGAG135100.075.973317
GAAACAC42550.073.093954
CGAGCTA7050.073.011795
AACAAGA141550.072.6256566
CAAGAGC136300.071.103138
AAGAGCG17000.070.3539059
CGGTGCA9750.067.6644442
GCGGTGC17250.066.827511
Produced by FastQC (version 0.11.5)