FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2817153
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT246570.8752453274635776No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC123460.43824385824980044No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT113180.40175311742031755No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA65670.2331076799875619No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC60270.21393939200320322No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA59130.2098927534287275No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT58940.20921831366631488No Hit
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG58870.20896983585911028No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA45320.16087163175021024No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA42680.15150046873563489No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT42500.15086152580282292No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG41880.14866072236758174No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT38840.13786968616897982No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC38040.13502993980092667No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG35470.12590725459355598No Hit
GTAAGAAGTACAGTAAAAATGAATTTAATGCGATTTTTCTACCTCCAAAA35040.12438089092072742No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC32910.11682006621578594No Hit
GTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTCTTAGTGGAG32830.11653609157898062No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT32400.11500972790615205No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT31880.11316389276691753No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGAGGGT31790.11284442130051155No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA30600.10862029857803251No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG30540.10840731760042852No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT30100.10684545709799928No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA29600.10507061561796607No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC29550.10489313146996276No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA29510.10475114415156012No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG29110.10333127096753354No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT28790.10219537242031228No Hit
AGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATTGTTCAAACA28690.10184040412430564No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCGA100.007088895145.018111
CGACCTT100.007091529145.000085
GCCGCAC100.007091529145.000083
CAAGCGT100.007091529145.000086
TCAAGCG100.007091529145.000085
ACGACCT100.007091529145.000084
CCCGAGG100.007091529145.000084
AGCCACC100.0070945406144.97949145
AGCACGC32700.0130.826741
GCACGCA33400.0128.503072
CACGCAC33600.0127.953953
GAAACAC16550.0127.477424
CGGTGCA5850.0123.9316942
AAACACG18100.0116.560855
GTAACAA62750.0108.374574
ACGCACT41550.0105.042184
CGCACTT41650.0104.7899865
AACACGT5000.098.600066
TAACAAG69450.097.815035
GCGGTGC11350.096.465791
Produced by FastQC (version 0.11.5)