FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2520374
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT184840.7333832201093965No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC85200.3380450679145238No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC78280.3105888253092597No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA66580.26416714344775816No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG60270.23913117656347826No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT43730.17350599553875734No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA42660.16926059386424397No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT42300.16783223442235162No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA41150.1632694195385288No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT39770.15779404167794145No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT38820.1540247598173922No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAACCCTGGAAATTATTCAAT32460.12879040967729394No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG32270.12803655330518407No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA32120.12744140353772893No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA31090.12335470846787024No Hit
GTATTGTCTCATGGTCATGTACCTGAACCCTGGAAATTATTCAATGCAAG30310.12025992967710347No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC30180.11974413321197569No Hit
GTAAGAAGTACAATAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA29430.11676838437469994No Hit
AAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACA29170.11573679144444436No Hit
GCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACAAT27660.10974561711872921No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT27580.10942820390941979No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG27410.1087537008396373No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT26850.1065318083744714No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT26490.10510344893257906No Hit
GCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACAAT25980.10307993972323157No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA202.130424E-6145.011183
CTGCGCA100.0070944894144.97954145
CTTTGCG100.0070944894144.97954145
CCGCACA100.0070944894144.97954145
AGCACGC31300.0122.087811
GCACGCA31200.0121.539842
CACGCAC31650.0119.811773
CGGTGCA5800.0112.508682
CAGCGTC301.5993204E-596.6741262
GTCTCGC406.1144783E-790.639181
GCGTCAG353.4369772E-582.863544
GCGGTGC11350.081.774911
TGTCGTC950.076.321682
GCAGCGT406.6599845E-572.511351
CGTCCAG1000.072.50565
CGTCAGA406.662604E-572.505595
ACGTGCA8650.072.086492
CAAGAGG34900.069.166468
TAACAAG82650.068.6895145
CGCACTT66900.062.2095875
Produced by FastQC (version 0.11.5)