FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3554922
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTA190190.5350047061510773No Hit
GGCTCTTGTTACTACTAATAACCCGGGCTCTTGTTACTACTAATAACCCG167420.47095266787850765No Hit
GGGCTGTTGTTTCTACTAATAACCCGGGCTCTTGTTACTACTAATAACCC127290.35806692805074203No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGGATTCCGGGTTA118860.3343533275835588No Hit
GGGCTCTTGTTACTACTAATAACCCGGGCTCTTGTTACTACTAATAACCC113880.3203445814000982No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTA110750.31153988751370637No Hit
ACAACAGCCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGAA104110.29286155926909224No Hit
GGCTCTTGTTACTACTAATAACCCGGAATCCTCTTGTTACTACTAATAAC96320.27094827959657064No Hit
GGCTCTTGTTACTACTAATAACCCGGGCTGTTGTTTCTACTAATAACCCG89110.250666540644211No Hit
ACAACAGCCCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGAA82350.2316506522505979No Hit
CCCGGAATCCTCTTGTTACTACTAATAACCCGGGCTCTTGTTACTACTAA81170.22833131078544058No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACAACAGCCGGGTTATTAG77670.21848580643963497No Hit
CAAGAGGATTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAG77220.21721995588088852No Hit
GGCTGTTGTTTCTACTAATAACCCGGGCTCTTGTTACTACTAATAACCCG76090.21404126447781413No Hit
GTAGAAACACGTGCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGT73330.206277381050836No Hit
GGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGGATT71730.201776579064182No Hit
CACGTGCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGTAACA70320.19781024731344315No Hit
GTAGTAACAAGAGCCGGGTTATTAGTAGAAACACGTGCGGGTTATTAGTA70200.1974726871644441No Hit
GGGCTGTTGTTTCTACTAATAACCCGGGCTGTTGTTTCTACTAATAACCC66910.18821791307938682No Hit
TGTTTCTACTAATAACCCGGAATCCTCTTGTTACTACTAATAACCCGGGC65780.18503922167631245No Hit
TGTTTCTACTAATAACCCGGGCTCTTGTTACTACTAATAACCCGGGCTCT64330.18096036987590727No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTA64160.18048215966482528No Hit
GTAGTAACAAGAGGATTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTA63820.1795257392426613No Hit
CCCGGGCTCTTGTTACTACTAATAACCCGGGCTCTTGTTACTACTAATAA61960.17429355693317602No Hit
GGGCTCTTGTTACTACTAATAACCCGGAATCCTCTTGTTACTACTAATAA57880.16281651186720836No Hit
GGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGCCCG57600.16202887151954387No Hit
GTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTA57120.1606786309235477No Hit
GGGCTCTTGTTACTACTAATAACCCGGGCTGTTGTTTCTACTAATAACCC55400.15584026878789464No Hit
ACAACGCACTTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTA53680.15100190665224159No Hit
ACAACAGCCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGTA53130.1494547559693293No Hit
GGCTCTTGTTACTACTAATAACCCGGGCTCGTGTTTCTACTAATAACCCG48690.13696503045636446No Hit
GGTTATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGTAACAAGAGC48630.13679625038186494No Hit
GCTCTTGTTACTACTAATAACCCGGGCTCTTGTTACTACTAATAACCCGG48250.1357273099100346No Hit
GGGCTCTTGTTACTACTAATAACCCGCACGTGTTTCTACTAATAACCCGG48210.13561478986036823No Hit
ACAACAGCCCGGGTTATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTA47220.1328299186311261No Hit
CAACGCACTTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAG45950.12925740705421948No Hit
GGGCTCTTGTTACTACTAATAACCCGGGCTCGTGTTTCTACTAATAACCC44920.12636001577531095No Hit
GGGCTCTTGTTACTACTAATAACCCGGCTGTTGTTTCTACTAATAACCCG43070.12115596347824228No Hit
CCCGCACGTGTTTCTACTAATAACCCGGCTCTTGTTACTACTAATAACCC42240.1188211724476655No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACAACGCACTTCCGGGTTA42130.11851174231108305No Hit
ACAACAGCCCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGAA41320.11623321130533946No Hit
GAATCCTCTTGTTACTACTAATAACCCGGGCTCTTGTTACTACTAATAAC41160.11578313110667407No Hit
ACAACAGCCCGGGTTATTAGTAGAAACAACGCACTTCCGGGTTATTAGTA40570.1141234603740954No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGCCGGGTTATTAG38140.10728786735686466No Hit
ACAACAGCCCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGTA38080.10711908728236513No Hit
CTAATAACCCGGGCTCTTGTTACTACTAATAACCCGCACGTGTTTCTACT38010.10692217719544901No Hit
CAAGAGGATTCCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAG37660.10593762676086844No Hit
CGGCTGTTGTTTCTACTAATAACCCGGGCTCTTGTTACTACTAATAACCC36690.10320901555645946No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGAGCCGGGTTATTAG36580.10289958541987701No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGTGCCCGGGTTATTA36500.10267454532054432No Hit
GGGCTCGTGTTTCTACTAATAACCCGGGCTCTTGTTACTACTAATAACCC36080.10149308479904763No Hit
CCCGGGCTGTTGTTTCTACTAATAACCCGGGCTCTTGTTACTACTAATAA35700.1004241443272173No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC6350.060.518381
CTCGCTC6800.056.5079233
CAGGATT9100.055.7697945
ACAGGAT9450.053.7042474
TCGCTCT7350.053.2658464
TCTCGCT7500.051.233852
ACGAGGA7500.045.4337924
GCACCTA4650.045.2155086
CTGTCGT7400.044.07969
TAAGGGT7400.040.1693234
GTCGTCC6200.039.7584653
TCGTCCA6350.038.819294
GATTGGG90050.038.243032
GCACGCA55550.037.8491632
CCTATAG5600.037.537639
ATCGCTT3100.037.419733
CTGTCGC3100.037.412369
CACGAGG9500.036.631953
CGGTGCA11200.036.2503622
CAGTCGG800.002038901736.244247145
Produced by FastQC (version 0.11.5)