FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2684151
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA110340.4110797045322711No Hit
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG75200.28016307577330785No Hit
GTAGTAACAAGAGCCGGCAGCGATAGTTCTGTGACATTATGTACTGAGTA69280.2581076847017921No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA54240.20207506954713056No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC42560.1585603790546806No Hit
AAATATAGTCATAACATTGTGCAGCCGTCCCTACCATCCATCTCCATAAT42210.15725642856903355No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT37730.1405658623527514No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA33070.12320469302956503No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC30600.11400252817371304No Hit
ACAACAGCCAACACCACAGCCATCTCAATTGGTTAAATTTACACAATTTT30140.11228876467829121No Hit
GTAGTAACAAGAGCCAACACCACAGCCATCTCAATTGGTTAAATTTACAC28550.10636510390063748No Hit
CACGTGCAGTTTGATATTACTGAACCTAAAGGCAAAGCTGCAATGTGGAG27580.1027512982689871No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA107700.0121.648121
TAGTAAC110700.0118.333782
AGCACGC42200.0117.518591
GCACGCA42650.0116.261332
GTAACAA120000.0109.102494
AGTAACA122450.0106.860363
CACGCAC47200.0105.3611153
TAACAAG124750.0105.180745
CGCACTT51950.0101.309295
ACGCACT54650.099.487684
CGGTGCA9850.084.636922
ACAAGAG155150.084.562237
AAACACG47250.083.463255
GAAACAC47200.083.0909044
AACAAGA158050.083.019926
CAAGAGC153750.079.769148
AAGAGCG10600.077.272029
GCGGTGC21250.073.3571551
GAAGCGG12750.072.219831
ACGTGCA27350.070.770482
Produced by FastQC (version 0.11.5)