FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3949708
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT239480.6063233028872007No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC204210.517025562396005No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT172430.4365639181428096No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT101910.258019073815077No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC100960.25561383271877314No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT89280.22604202639790077No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT85630.2168008369226282No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT76740.19429284392669027No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG58140.1472007550937943No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT54780.13869379711107757No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT54070.13689619587068208No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGT52890.13390863324579944No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA51720.1309463889482463No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT50850.1287436944705786No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC50320.12740182312211434No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG48500.1227938875481428No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA48150.12190774609160981No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC48070.1217051994729737No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCCT46640.1180846786648532No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC46300.11722385553564972No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC44960.11383119967349484No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA44540.11276782992565527No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA44460.11256528330701916No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT43900.11114745697656638No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAACA41160.10421023528827952No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC41030.10388109703299586No Hit
CAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAA40650.10291900059447433No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA39820.10081757942612467No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC39560.1001593029155573No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC39550.10013398458822778No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA39550.10013398458822778No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCGC100.007090512145.007781
GACGCGG100.007092928144.991243
GTAAACG100.007096419144.96739145
AGCACGC32950.0130.705031
GTAACAA94000.0129.103914
GCACGCA34050.0126.468142
CACGCAC34200.0125.913463
CGGTGCA7750.0123.476422
TAACAAG101650.0119.173835
GAAACAC32850.0116.301964
GAGCGAA256.4655305E-6116.006221
GTCTCGC203.8660862E-4108.755831
AAACACG35350.0108.076935
CGCACTT41550.0105.908175
ACGCACT41600.0105.606614
CTCTCGT352.7642636E-7103.565185
TAGAAAC40500.097.913842
TAGTAAC130650.093.664452
AGTAACA137350.089.148253
ACGTGCA26450.087.981462
Produced by FastQC (version 0.11.5)