FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2747799
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT75310.27407390424117634No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA70150.25529523811603394No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA69290.25216546042851024No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC58520.21297045380684687No Hit
CACGTGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG56460.20547354446231328No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG55320.2013247693881539No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT54810.19946873843392476No Hit
CACGAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG54730.19917759632345744No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT46570.16948110105579048No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC35500.12919431151987465No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG34820.1267196035809024No Hit
ACACGTGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATA34220.12453603775239745No Hit
ACACGAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATA30110.10957861182713874No Hit
ACAACAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAATCCTGAG29900.10881436378716201No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT29090.10586654991868037No Hit
AACACGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACC28830.10492033805966157No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC31800.0119.491961
GCACGCA33450.0113.5791242
GTAACAA101650.0104.2805564
CGCACTT38950.0102.566965
CACGCAC37550.0100.984583
TAGTAAC105300.0100.597042
TAACAAG106400.0100.102185
GTAGTAA108300.098.094281
ACGCACT41550.097.195814
AGTAACA116700.090.707953
AAACACG37800.082.8619845
CGGTGCA8850.081.925692
GAAACAC38700.081.3096544
ACAAGAG136300.078.288197
GCGGTGC15500.073.919651
AACAAGA144500.073.808816
GAAGCGG8400.073.3793951
CTCGCTC13350.069.5171663
CAAGAGC138050.068.473978
GTCTCGC13600.068.250461
Produced by FastQC (version 0.11.5)