FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2454012
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC123320.5025240300373429No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT89420.36438289625315606No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC74810.3048477350559003No Hit
TTCGAATATATTGCTAAAATTCTCACCAACAGAAATAACTGCACCAACAA64170.2614901638622794No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT58040.2365106609095636No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT46950.19131935785155083No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT45830.18675540298906446No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA38040.15501146693659199No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA36610.14918427456752453No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC36380.14824703383683535No Hit
ATATCCGAATATAGTAAGTATGATAATGAAGCTGACACATATAGTGATAA35730.14559831003271378No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG35690.14543531164476783No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA34870.142093844691876No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC33230.13541091078609233No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT32260.1314581998784032No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA31660.12901322405921406No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC29790.1213930494227412No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC28660.1167883449632683No Hit
ATTCGAATATATTGCTAAAATTCTCACCAACAGAAATAACTGCACCAACA27690.1128356340555792No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA27180.11075740460926842No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC25280.10301498118183612No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA25000.10187399246621451No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC24550.10004026060182265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA55300.0130.0554
AGCACGC8100.0123.531811
CACGCAC8200.0122.8970263
GCACGCA8300.0121.416342
GAAACAC17400.0120.000674
CGGTGCA7200.0113.7853552
GGTGCAT7050.0113.121213
AAACACG18700.0111.658385
TAACAAG66450.0108.450565
TAGAAAC21900.0100.639842
CGCACTT10700.098.2482155
ACGCACT10800.098.009814
TAGTAAC78050.092.5181352
TCGTCCA750.087.0004964
GTCGTCC750.087.0004963
GCACTTA750.087.0004966
CTTAGCG259.3973015E-486.979229
ACGTGCA13950.085.7531742
AGTAACA87150.083.190373
AACACGT7400.081.318026
Produced by FastQC (version 0.11.5)