FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2873906
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT57530.20018052086602695No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC56290.19586583555620818No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA54010.18793238192202527No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA42040.14628175034256513No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC38850.13518187442456364No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC32550.11326048938274252No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA32260.11225140975383328No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC31960.11120753427565132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC31250.0110.463741
GCACGCA31700.0108.8747942
TAGTAAC111850.097.1729352
GTAACAA113050.095.949054
GTAGTAA114200.095.763071
TCCGTCG406.126429E-790.610048
AGTAACA122550.088.688633
CACGCAC40100.087.333813
CGCACTT40500.085.576115
TAACAAG128450.084.614965
ACGCACT46800.079.7886354
GTCTCGC11900.075.567931
ACAAGAG145700.073.9805767
ACGTGCA18150.073.505682
CCGTCGC502.3096054E-672.488049
CGGTGCA7000.069.399512
CGCTCTG12450.068.139065
AAGAGCG18600.067.8113869
AACAAGA161750.066.657086
GCGGTGC13950.066.542421
Produced by FastQC (version 0.11.5)