FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365739
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC48350.3540207902095496No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT38940.2851203634076496No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC30160.22083282384115852No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA27940.20457788786876555No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT27730.20304025879029597No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT25960.19008024227176643No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG24980.18290463990557493No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT19170.14036356873458253No Hit
AGCACAGCCACTATGCTTGATTAGCATTATAGAGATGGTACAATGATGGT16230.11883676163600805No Hit
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG15890.11634726693753344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA4450.0115.6897352
GCACGCA11050.0110.897282
CACGCAC11200.0110.706863
AGCACGC11100.0110.409871
TAACAAG43400.093.059765
GTAACAA43800.092.375454
CGCACTT13000.090.9160845
AAACACG12400.090.0524445
GCGGTGC10650.089.8811341
ACGCACT14350.087.921254
GTAGTAA50200.081.618561
TAGTAAC50300.081.1590352
GAAACAC13900.080.334554
ACGTGCA8600.080.097992
AACACGT5650.078.2848746
GAAGCGG4100.077.8239061
CCACTAT13500.076.24448
AGTAACA54000.075.5981453
CGGTGCC3350.073.5919952
ACAACGC4100.068.9499748
Produced by FastQC (version 0.11.5)