FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2939735
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC80990.2755010230513975No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC80590.274140356188568No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA73380.24961433598606678No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA50110.17045754124096218No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA48180.16389232362781No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA48150.16379027361309778No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA42040.14300608728337758No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA41320.14055688693028454No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT40540.13790358654776708No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT39900.1357265195672399No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA39610.13474003609168855No Hit
GTCTCGCTCTGTCACCCAGGCTGGAGTGCTGTGGCGCTATCTCGGCTCAC39310.13371953594456643No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG37430.1273244016892679No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT36350.12365060115962832No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT35850.1219497675810915No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA35420.1204870507035498No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA34710.1180718670220275No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT33890.11528249995322708No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG33610.11433003314924645No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG32570.11079229930588982No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA31620.1075607155066698No Hit
GTCTCGCTCTGTCACCCAGGCTGGAGTGCTGTGGTGCGATCTCGGCTCAC30220.10279838148676668No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG29920.10177788133964456No Hit
GTCACCCAGGCTGGAGTGCTGTGGCGCTATCTCGGCTCACTGCAACCTCT29920.10177788133964456No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG29450.10017909777581993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13200.092.8340151
GTAACAA92250.088.571264
TAGTAAC96600.085.3333052
GCACGCA4200.084.5827942
CGCTCTG14850.082.50795
CGCACTG2800.080.267355
TCTCGCT15350.079.8203352
CGGTGCA4000.079.749492
AGTAACA106900.077.1791153
CACGCAC4650.076.397373
GAGCGGT300.001936165572.499544
TAACAAG112850.072.14625
AGCACGC5200.068.326171
ACAAGAG128750.062.9983867
CTCGCTC20050.061.109343
ACGTGCA5650.059.0261732
CTGTCGC7650.057.798299
TCGCTCT21200.057.7944454
TCTGTCA20600.056.6506968
AAACACG30900.055.137195
Produced by FastQC (version 0.11.5)