FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547319
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC78490.5072645007267409No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT60230.38925392889249083No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC48330.31234671066535086No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT40520.2618723094591355No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT34570.22341870034556544No Hit
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC25150.16253920490861937No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT19940.1288680614663169No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC19810.12802789857812125No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT19290.12466724702533867No Hit
GAATAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCT19210.12415022370952597No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG17870.11549008316966314No Hit
CTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAAGCT17710.11445603653803772No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC17680.11426215279460795No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA17470.11290496659059962No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT17250.11148315247211467No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT16160.10443870979416656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC8500.0123.667431
GTAACAA50650.0120.224244
TAACAAG51600.0118.010815
GCACGCA9100.0117.1030352
CGGTGCA5050.0107.661912
CTCGCTC9750.0105.578613
CGCACTT10400.0104.556295
TAGTAAC59300.0102.931812
CACGCAC10850.098.883583
GTCTCGC10450.098.5095441
AGTAACA63500.096.009563
ACGCACT11900.094.422814
AAACACG14850.093.727495
ACAAGAG66250.091.799487
GAAACAC15550.089.508244
TCGCTCT11600.088.740654
TCTCGCT12850.079.544142
AACAAGA80400.076.098946
CTGTCGT12100.074.883969
CAAGAGC58700.074.463498
Produced by FastQC (version 0.11.5)