FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2827260
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC96690.34199189321109486No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA72350.2559014735114563No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC67180.23761521756046491No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA60090.21253793425436643No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT51320.18151850201254927No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT50930.18013907458104314No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT34610.12241534206263308No Hit
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG29350.10381075670437101No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA110750.0117.118434
AGCACGC24800.0115.783911
GCACGCA25250.0114.282652
TAGTAAC118250.0110.058082
TAACAAG118600.0109.549885
AGTAACA127550.0101.919783
CGCACTT30550.097.541465
CACGCAC30950.094.172483
ACGCACT33050.093.2343754
ACAAGAG145800.089.040827
GTAGTAA148950.088.356981
AAACACG38000.083.760555
CGGTGCA10250.081.345332
CAAGAGC136850.079.8744058
AACAAGA165450.078.528966
GAAACAC43600.073.002324
CTTAGCG3300.072.485499
ACGTGCA21600.071.8321152
AAGAGCG16050.070.0015649
CGAGCTA5550.069.237525
Produced by FastQC (version 0.11.5)