FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2799344
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT75320.26906303762595807No Hit
AGTAGAAACAACGCACTTTTTCCCAGTTTATTTGCTGACATTGATTACAA68210.2436642299052921No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT58620.20940620373916174No Hit
GTAAGAAGTACAGCAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA58270.20815591081339058No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG55960.19990397750330074No Hit
GGATAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATA55390.19786778616704484No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA50860.18168542344206356No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC48540.17339776747695174No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA48110.1718616933110043No Hit
CTATTATCCAGGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT47080.16818225984373483No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT44190.15785841254236707No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG37310.13328122588720787No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC37190.13285255402694346No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCCGGAGT35410.12649392143302143No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC35050.12520790585222824No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA33830.12084974193954012No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCGGGCACTGAAG33200.118599214673152No Hit
TACCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATT31070.11099028915345882No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT31040.1108831211883927No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA30890.1103472813630622No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC29110.10398864876914021No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT29040.10373859018398596No Hit
ATTACATCCTTTGCAATTCCTTCTCCATTCTTTTGGCTTGCCCCCAGAGA28110.10041638326693683No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA11250.0120.525362
AGCACGC12200.0111.756481
CACGCAC12250.0110.686553
CGGTGCA3650.095.353742
GACGCAC259.384118E-487.0102844
GCGGTGC6300.079.4297561
GTAACAA55100.077.114374
TAACAAG57550.073.57955
GAGCACG608.0193786E-872.522821
AGCGTCA300.001935199872.508573
CGCACTG554.060663E-665.916885
ATGCCGG350.003569039362.13466145
AAACACG18450.059.7360575
GAAACAC19100.058.082784
GAAGCGG4500.056.4066351
ACGGACC651.09339035E-555.775824
ACGCACT26300.054.8638954
CGCACTT25400.054.5241555
TAGTAAC80200.054.426632
AGCGGTG3200.054.3921171
Produced by FastQC (version 0.11.5)