FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2511830
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT97300.38736697945322734No Hit
TAACCGTTACACAGCAAAAAGACCCTTTGTCAAATTAAATGTGGAGACTG67520.26880800054143794No Hit
TAACCGTTACACAGCAAAAAGACCTTTTGTCAAATTAAACGTGGAGACTG58790.23405246374157487No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC48940.19483802645879697No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC46780.18623871838460404No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT43480.17310088660458708No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA41770.16629310104585102No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT39350.1566586910738386No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT36400.1449142656947325No Hit
GTACCTGTTGTCTCTACAGGCACATTCTATGGTTTTATTGCTGGCAAATC35610.14176914838981935No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG35200.1401368723201809No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA33620.1338466377103546No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA30210.12027087820433709No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAACCCTGGAAATTATTCAAT30010.11947464597524514No Hit
GACTCAACCATTCCTCCATTACAGAGCCATATCAACACCTGTGACAGTGT28450.11326403458832802No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC28360.11290573008523666No Hit
GTCTCTTTTCCACCATCATGTACCATCTCTATCCCAATACAGGGGACATC28100.11187062818741715No Hit
GTATTGTCTCATGGTCATGTACCTGAACCCTGGAAATTATTCAATGCAAG27370.10896438055123156No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG27330.10880513410541318No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA26930.10721266964722931No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA26170.10418698717667996No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA26040.10366943622777018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCTA2150.080.929315
CGAGGAT809.822543E-1172.499175
GCACGCA32600.071.164832
CGGTGCA7700.067.7914352
CACGCAC36200.064.488223
AGCACGC40800.060.4256061
ACCGTTA31750.057.314313
TAACCGT31850.056.91581
GTCTCGC651.0931652E-555.777481
CGTTACA33400.054.482915
ACGTGCA12000.053.770222
GTTACAC33900.053.679336
CCGTTAC34050.053.4428564
AGCATAG9550.053.1407555
AACCGTT35000.052.4065482
GTAACAA162500.050.2809644
TAACAAG162900.050.1129955
ACCTGTT22950.049.2804833
CGTCAGA604.9467967E-448.3327872
TAGTAAC172050.047.8271182
Produced by FastQC (version 0.11.5)