FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3867272
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA153220.3961965954295431No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT123510.31937241549081624No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC107830.2788270388015118No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT94440.24420314888634673No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT66160.17107666592885115No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA59860.15478611279475557No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG58140.15033853320893903No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC55720.1440808921637785No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC54800.14170195424578358No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA54240.14025390507830843No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC53040.13715094257657595No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA51800.1339445479914524No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC51690.1336601097621269No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT48920.1264974379872944No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG48140.12448051236116828No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC47480.12277388298521541No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA47180.1219981423597823No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT46910.12129997579689247No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA46850.12114482767180586No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGT45460.11755056277396572No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG44810.11586979141886063No Hit
GTAGAAACACGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA44140.11413730402205999No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAC41250.10666433599705424No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA40730.10531971891297018No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT40310.1042336820373638No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA40010.10345794141193068No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT39680.10260462672395425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA8000.0115.092782
AGCACGC13650.0114.734691
GCACGCA14250.0109.385042
CACGCAC15050.0103.088833
GTAACAA97500.099.4171144
TAACAAG99300.097.6149755
GAAACAC25450.087.739924
GCGGTGC20900.083.9542541
AAACACG26750.083.7469565
ACGTGCA10800.078.5412
TAGTAAC124700.078.487712
CGCACTT20650.075.834715
AGTAACA130550.074.915113
ACAACGC6900.074.588288
ACGCACT21250.074.375844
GTAGTAA134850.073.500651
CAACGCA7450.069.0817649
AACACGT9250.065.05356
GAGCACG350.003566117562.1479571
GCGTCAG350.003566117562.1479571
Produced by FastQC (version 0.11.5)