FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2874432
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT139310.48465227217064105No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC115750.40268825284438803No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT103410.3597580321955781No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA65420.22759279050608958No Hit
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG54220.1886285707924209No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC45520.1583617215505533No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT40190.13981892770467347No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA40150.13967976977712465No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG32140.11181339478547414No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA31570.1098303943179035No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT31300.108891078306949No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG30720.10687328835749116No Hit
GTATTGGGATAGAAATGGTACATGATGGTGGGAAAACGACTTGGCACTCA30000.10436844566161245No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG29830.10377702446952998No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC21700.0121.94991
GCACGCA22250.0119.574632
CACGCAC23700.0112.564763
GTAACAA64250.0108.3181154
CGGTGCA7800.0100.376532
GAAACAC18450.0100.1951754
TAACAAG73250.095.405275
AAACACG20000.092.792525
CGCACTT29600.090.86265
ACGCACT30600.088.840874
ACGTGCA10650.086.448432
GCGGTGC13300.084.494491
TAGTAAC86900.080.752982
AGTAACA91550.076.651393
ACAACGC7850.076.640548
GACGATT300.001937668172.485327
GTAGTAA101000.070.635341
AACACGT7200.070.480436
GAAGCGG6350.066.2220461
CGTCAGA451.194714E-464.439252
Produced by FastQC (version 0.11.5)