FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3070798
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC83660.27243732736572057No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT73180.23830939058837475No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC71500.23283849995994524No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT65490.21326704003324218No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT65260.21251804905435004No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG60990.19861286870709177No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA60140.19584485856770784No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT50750.16526648773380728No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT46040.1499284550791032No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT44330.14435986997516606No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG42940.13983335927664406No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC42100.13709791396242932No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC41310.1345252927740607No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA41090.13380886662033778No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC40660.1324085791380612No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT40620.13227831983738428No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA39940.1300639117258771No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC39540.1287613187191082No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC38990.12697025333480091No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT37770.1229973446641557No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA37340.12159705718187912No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA37040.12062011242680241No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG36320.11827544501461834No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA36030.11733106508471088No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA35210.1146607494208346No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA34940.11378149914126555No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA34240.11150196137941994No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT34070.11094835935154315No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC33370.10866882158969754No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA33240.10824547886249763No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT32720.10655210795369803No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG32650.10632415417751347No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC32270.10508669082108298No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT32230.1049564315204061No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA32110.10456565361837541No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT32020.10427257019185242No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGGG100.007093097144.989583
CCGTACT100.007093097144.989582
CGGGCCG100.007093097144.989585
CGTACTA100.007093097144.989583
CCGTAAT100.007096551144.96597145
CGGTGCA6800.0113.0065842
GGTGCAT6950.0110.567593
TATACGC259.397371E-486.97958145
TTCGGAA353.4424178E-582.8377145
CACGCAC5300.082.069573
GCACGCA5300.082.069572
GAGCGAA554.3923137E-879.1045461
GTAACAA92250.076.620514
AGCACGC5850.074.37181
ACGTGCA9350.072.8824542
GGCTCGG300.001934801372.5125051
AGCGAAG608.0412065E-872.494792
GCTCGGG300.001936677572.494792
TAACAAG101000.069.695495
GGTGCGT3500.068.352233
Produced by FastQC (version 0.11.5)