FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718546
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC96880.3563669696963009No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT83130.30578846192045306No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT61820.22740097096021183No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC54590.20080587196243874No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT49360.1815676468229708No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT41920.1542000760700757No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT40620.14941810806217737No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC30840.11344299489506524No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT30720.11300158246356692No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA30530.11230267944702793No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA30250.1112727171068652No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG30230.11119914836828215No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA29730.10935992990370588No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC29610.10891851747220757No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT29140.10718965211550587No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC28930.10641718036038382No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA28540.10498258995801431No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT27920.10270195906193973No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA27240.10020062195011598No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAC100.0070900694145.009981
CGCCCCC100.00709553144.97263145
AGCACGC7900.0125.73651
CGGTGCA4700.0124.9347152
GTAACAA52650.0121.165854
GCACGCA8200.0121.1163252
CACGCAC8500.0116.841633
TAACAAG59400.0107.64115
GAAACAC19800.091.8976754
AAACACG19950.091.206715
TAGTAAC73950.086.658282
CAGCCTT2600.086.433945
CGTGCAC353.4399927E-582.849123
AGTAACA80750.079.540293
ACGTGCA10650.078.959492
CGCACTT13250.078.784835
ACGCACT13400.077.902914
CAACGCA4900.075.444949
AACACGT6200.074.831476
TAGAAAC27700.073.801522
Produced by FastQC (version 0.11.5)