FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2912466
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC144300.49545642764585063No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC89240.3064070104166023No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT85550.29373733461609514No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT66220.22736746111370912No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT55520.19062883480871537No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA45300.15553829641273065No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA44310.15213911510039946No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG42070.14444803819169047No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA41990.14417335687352228No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA40570.13929776347603715No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC36580.12559803273239928No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT36550.1254950272380862No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT35670.12247353273823626No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA33350.1145077745113591No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG32980.11323737341483128No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA32750.11244766462509778No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT32030.10997553276158419No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC31660.10870513166505635No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA30360.10424156024482346No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC29690.10194110420516497No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT29270.10049902728478204No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGTA100.007088923145.0184
AGCACGC11850.0124.222261
GCACGCA12150.0121.146722
CACGCAC12400.0118.7042543
GTAACAA65200.0116.993054
CGGTGCA6900.0110.339782
TAACAAG72850.0104.608035
GAAACAC20750.096.096264
CGCACTT16350.090.913425
AAACACG22050.090.101885
ACGCACT16750.090.041024
CAGACCG259.394184E-486.986899
AACACGT6800.081.039476
TAGTAAC95100.080.972192
GCACTTA1450.080.009936
ACGTGCA12600.078.263682
AGTAACA102400.075.3413853
CGGTGCG3500.074.580692
ACAAGAG105850.072.180917
TAGAAAC31000.070.172
Produced by FastQC (version 0.11.5)