FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2138333
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCAAAGAAGAACAGATAGCACTGATGGAATGCCTATTA61000.2852689454822986No Hit
GTAGTAACAAGAGCCATGGTGGTTTGCAGCACCCATCAACTTGTCATCTA46180.21596262135036964No Hit
CACGTGCACTGTGGGGATGGTTCTGATGCTTTCAAGGCCTAGAGTCTCAC41730.1951520179504315No Hit
GTAGTAACAAGAGCAGCCAACACTTACGTGGTACTTATGGGCCAGAGTAC39040.1825721251086711No Hit
CACGAGCACTGTGGGGATGGTTCTGATGCTTTCAAGGCCTAGAGTCTCAC37010.1730787487262274No Hit
GTAGTAACAAGAGCTATAGTGAGGGGTCCTAAGGCCAGGAACAATACCAG35190.1645674457626572No Hit
GTTTTGGGGAGTGTAAATTAGTTCAACCATTGTAGAAGACAGTGTGGTGA32670.15278256473617532No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA31360.14665629721844073No Hit
GTTCTACACTGTGCATATAACACAACACAAGAGTAAACACAGACACGTTC28160.1316913689308447No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG24660.11532347861628661No Hit
CACGTGCTCTCCCTGCCAATCGTGTCCGACTGATCTCTCAAACCCAAGCA23820.11139518494079266No Hit
ACAACAGCTATAGTGAGGGGTCCTAAGGCCAGGAACAATACCAGGAACTA22790.1065783486482227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC68600.0123.014972
GTAGTAA73800.0115.146611
GTAACAA77250.0109.428184
TAACAAG82950.0101.384295
AGTAACA87500.096.443743
AGCACGC9550.090.349041
AACAAGA101250.082.988436
CTTAGCG1000.072.483269
CAAGAGC115450.071.7612468
AAGCGGA6450.066.324231
CGAGCAC9000.065.2486653
ACAAGAG129850.064.752087
GTAGAAA49100.064.089681
AAACACG32100.063.4644285
AAGAGCG11950.061.8685539
AACAACG11350.059.3915677
AAGAGCA62550.059.330829
GCGGTGC5100.058.2899361
GAAACAC35600.057.02134
ACGAGCA15550.056.8798642
Produced by FastQC (version 0.11.5)