FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3584818
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC177880.4962037124339367No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT150930.4210255583407581No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT69250.1931757762876665No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC67160.18734563372533836No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT62590.1745974272613003No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG48260.13462329189375863No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA47440.132335867539161No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA46290.1291278943589326No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC45380.12658941123370837No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA44720.12474831358244687No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA44540.12424619604119373No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT44170.12321406553972894No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC43100.1202292557111686No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT42610.11886238018220172No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC42340.118109203870322No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC41450.1156265115830148No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA41390.11545913906926376No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC39190.10932214689839206No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC38710.10798316678838368No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA38450.10725788589546247No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT38210.10658839584045827No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT37270.10396622645835855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA68500.0127.439934
GCACGCA9150.0123.6155552
AGCACGC9100.0123.5186841
CACGCAC9550.0118.437943
CGGTGCA6800.0114.089192
GAAACAC23900.0112.2466664
GGTGCAT7150.0105.462233
TAACAAG83450.0104.5222855
AAACACG25950.0103.37945
CGCACTT11700.094.81465
TAGAAAC31100.093.254382
ACGCACT12250.092.333264
TAGTAAC102600.085.649512
AACACGT8500.084.447326
ACGTGCA16500.081.293792
AGTAACA115400.076.149393
GAAGCGG8400.073.380721
ACAAGAG119150.073.067067
GTAGAAA40150.072.968961
ACACGTG10450.068.673887
Produced by FastQC (version 0.11.5)