FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1777403
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG115490.6497682292648319No Hit
CAACAGCACTAAGAAGAGAACCTCTTCCTCCACCAAATCAATCCCTGCTT92870.5225039003534933No Hit
CACGAGCACTAAGAAGAGAACCTCTTCCTCCACCAAATCAATCCCTGCTT79510.4473380544536045No Hit
CACGTGCACTAAGAAGAGAACCTCTTCCTCCACCAAATCAATCCCTGCTT71620.40294744635853547No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG67980.382468129062458No Hit
AGAGCATAGAAAACCAAGCAAATGAGAAAAACAATGTAATTAACTCCAGG58870.33121357396156076No Hit
ACAACGCACTAAGAAGAGAACCTCTTCCTCCACCAAATCAATCCCTGCTT54000.3038140477989516No Hit
ATACTAAACTGTGAATGCAGGTTTCAGCCAATATCAAACTGCTTAGGGAG45290.2548099671261948No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA41220.23191138981986642No Hit
GTAGTAACAAGAGCTGTGCTTTTAGATGAAAATAAGTTCTATGGCTGCAT40440.22752296468499267No Hit
GTAGTAACAAGAGCATAGAAAACCAAGCAAATGAGAAAAACAATGTAATT38440.21627059254429076No Hit
ACAACAGCCACTCTTCTTCCTTCCGTTTTTAAGTTATAAAAAGCATTTAA33050.1859454496250991No Hit
AACACGAGCTGTGCTTTTAGATGAAAATAAGTTCTATGGCTGCATGGAAA32450.18256973798288853No Hit
ATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACA31800.17891271703716038No Hit
ATAATATGTTGTGGGAAAGACAGTGTAAGAGAAGCTAACTCCTCTTCCAT31670.17818131284801478No Hit
CAGTTATACTAAACTGTGAATGCAGGTTTCAGCCAATATCAAACTGCTTA30920.17396167329525156No Hit
ACATATTATCACCCCCACCCTCGACACCCACATTTCCTCTCCTATTAGTC29010.16321565790088122No Hit
CTCTTACACTGTCTTTCCCACAACATATTATCACCCCCACCCTCGACACC27930.1571393769449022No Hit
GTGTAAGAGAAGCTAACTCCTCTTCCATTGTAGGAAGTCAACAATGTCAA27620.15539525926309342No Hit
AACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCT26850.15106309598892317No Hit
CTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAA26700.15021916807837052No Hit
ATATCAAACTGCTTAGGGAGACTAATAGGAGAGGAAATGTGGGTGTCGAG26150.1471247657396775No Hit
CATATAAATATATCCTTTTATCATATCCTGGCAAACTTGAGCCTGAAGAG25230.1419486745549546No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA25000.1406546517587739No Hit
GTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCTAGCGCAAGTT24740.13919184338048265No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG24160.13592865545967908No Hit
ATATTATACTTACCATTGAATTTATCAGAGAAGAGTACTTAATTTCCATT24130.13575986987756855No Hit
ACAACAGCCAGAGTCAAAACACCTTCTTGTATTTGCCAAACATTCATTAA23260.13086508799636323No Hit
CCAATAAAGGGCTCAACAGGAAATGGTAAACATACAGTTATACTAAACTG23060.12973985078229303No Hit
GAAGAGAACCTCTTCCTCCACCAAATCAATCCCTGCTTCTCCAAACACAC22990.12934601775736848No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT22560.12692675774711756No Hit
GACTTAAATAATGCTATTTCTTAATCTTGACTTGACATTGTTGACTTCCT22540.12681423402571054No Hit
GATAATATGTTGTGGGAAAGACAGTGTAAGAGAAGCTAACTCCTCTTCCA22070.1241699265726456No Hit
ACATACAGTTATACTAAACTGTGAATGCAGGTTTCAGCCAATATCAAACT21610.12158188098028416No Hit
GTTATACTAAACTGTGAATGCAGGTTTCAGCCAATATCAAACTGCTTAGG21050.11843121678088762No Hit
GACTAATAGGAGAGGAAATGTGGGTGTCGAGGGTGGGGGTGATAATATGT20880.11747476514892795No Hit
ACACGAGCACTAAGAAGAGAACCTCTTCCTCCACCAAATCAATCCCTGCT20530.11550560002430513No Hit
TCCATATACAGAATCTCAAATGATTCTAAAAATGGAGCCCTTAGGTAATG20480.11522429072078758No Hit
GCTTAGGGAGACTAATAGGAGAGGAAATGTGGGTGTCGAGGGTGGGGGTG20360.11454914839234547No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG19800.11139848419294891No Hit
AACAACGCACTAAGAAGAGAACCTCTTCCTCCACCAAATCAATCCCTGCT19710.11089212744661735No Hit
GTAGTAACAAGAGCCGGCAGCGATAGTTCTGTGACATTATGTACTGAGTA19680.1107233418645068No Hit
ATAGAAAACCAAGCAAATGAGAAAAACAATGTAATTAACTCCAGGAAAAA19030.10706632091877868No Hit
AGAAACAACAGCTATGAGTGACTTGGCTAAGATTCACCCATGGGTCACTC18880.10622239300822606No Hit
ATTTAAGTCTTTACATGAAGCTAATCACTAAAGATCCCTGAGTTGAGTAG18630.10481584649063831No Hit
CTACTCAACTCAGGGATCTTTAGTGATTAGCTTCATGTAAAGACTTAAAT18600.10464706090852777No Hit
ACACGTGCACTAAGAAGAGAACCTCTTCCTCCACCAAATCAATCCCTGCT18290.10290294322671897No Hit
AACAAGAGCTGTGCTTTTAGATGAAAATAAGTTCTATGGCTGCATGGAAA17860.10048368321646807No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGCG203.8695603E-4108.7287757
GCACGCA5700.094.1097262
GTAGTAA73900.090.5617751
CGAGCCG4200.088.023485
GGTTCGA259.383693E-487.009951
GCACTAA51100.086.817686
AGTAACA81200.082.309993
TGCACTA12650.081.945145
TAGTAAC84250.079.416272
AGCACTA34650.079.289125
ACAGCAC20350.076.58643
GTAACAA87850.075.666784
GCGGTGC5700.075.052441
ACTAAGA59700.074.914198
CACTAAG60050.074.1154257
ACGTGCA22750.073.286522
AAGAGCG5450.073.1508569
CACGCAC7350.072.9830553
CGTGCAC18250.072.688543
GTCTCGC502.3054126E-672.5082861
Produced by FastQC (version 0.11.5)