FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402593
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTAGTCATAGTATGGCTGTGGTGTATCTGTGCCACATTTTCTTAATC61850.25743020145317996No Hit
CCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCATGTCCTTTGTA53620.22317554408924026No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG47350.19707873951185242No Hit
ACAACAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAATCCTGAG45510.1894203470999874No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT38090.15853704726518392No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA36960.1538337954035494No Hit
TCCCCTTCCTGTGTCCATGTGTTCTCATTGTTCAATTCCCACCTATGAGT32910.13697700775786825No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG31650.13173267382365636No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT29440.12253427858984023No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC28310.11783102672820572No Hit
GGGTATATACCCAAAGGATTATAAATCATGCCGCTATAAAGACACATGCA26520.11038074280579357No Hit
CACATATGCTTATTGTGGCACTATTCACAATAGCAAAGACTTGGAACCAA26110.10867425319228018No Hit
GGTTAGTTACATATGTATACATGTGCCATGCTGGTGTGCTGCACCCATTA24860.10347154095595884No Hit
GTATTCTAGTCATAGTATGGCTGTGGTGTATCTGTGCCACATTTTCTTAA24810.10326343246650597No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC24810.10326343246650597No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA100.0070945024144.979317
TAGTAAC79350.098.313622
GTAGTAA82100.095.483941
AGTAACA88100.088.384633
GTAACAA88150.088.334494
AGCACGC10450.084.662551
TAACAAG97800.079.3960655
AACAAGA103850.074.4914256
CAAGAGC107600.070.5359348
ACAAGAG112900.068.573037
GCACGCA13550.066.883512
AAGAGCT43000.066.420759
AGTCTCG8350.062.516462
CGGTAGC350.00356354262.1585661
AAGAGCG10850.060.129679
GGTTAGT9650.059.367341
CTCGCTC14450.058.202083
CGCTCTG15100.056.6569825
TTTCGAC400.00605134554.3762972
GCGGTGC7800.053.9238851
Produced by FastQC (version 0.11.5)