FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3923464
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA149480.38098985998087403No Hit
GTATATATGGAGCTATTTAATTCAAAGCAGTAGGATCCAGTTACTATGTG143150.36485615772184987No Hit
CTTCTACACTGCTGGTGGGAATGTAAACTGGTACAGCCACTATGGAAAAC108760.2772040217522067No Hit
GTATACACAACACCATATTTGTAGTCTTTTATCCCTTGCCCCCCTCCAAC105540.26899698837557834No Hit
CTATTATTCCAAGTGAAATAACTCAGGAATGGAAAACAAAACATCATATG102990.26249762964563966No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA99170.2527613353913786No Hit
GAATATATACAAATGGCCATCAAACATATGAAAAAATGCTCAACATCACT86700.22097819681791397No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT79960.20379949962584082No Hit
ATGGAAAACAGTGTGGAGATTCCTTAAAGAACTAAAAGTAGAACTACCAT79430.20244865251726535No Hit
AGTAGAAACAACGCACTTGCAGCCTTTCAGAAGCAGTTTTATGAAATGAG76970.19617868291897161No Hit
CACATATATCACAGTTTCTTTATCCATTCATTCATTGATGGGCATTTGGG76340.19457295899745736No Hit
AATTAGAATAGTGGTAAATGCCTAACTGGCATTCCAAAGGATGGGGTAAA76270.1943945452284002No Hit
GTATATATATATATGATTGGAGACTATTATTCCAAGTGAAATAACTCAGG75890.19342601333923287No Hit
CTAATATGTGGGAGCCAAGCTGTGAAGACACAAAGGCATAAGAATGATAC71850.18312899009650657No Hit
CACTAATATGTGGGAGCCAAGCTGTGAAGACACAAAGGCATAAGAATGAT71450.18210948284475148No Hit
GTTTATAGCAGCACAATTTACAATTGCAAAATCATGAAACCAACCCAAAT69300.1766296313665679No Hit
TGTGTATACTGCTTGGGTGATGGGTGCACAAAAATCTCACAAATCACCAC69030.17594146397163324No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT68310.17410635091847407No Hit
GGAATAGACAATTCTCAAAAGAATATATACAAATGGCCATCAAACATATG68260.1739789125120047No Hit
GTATCATTCTTATGCCTTTGTGTCTTCACAGCTTGGCTCCCACATATTAG67970.17323976975448227No Hit
GTGTATATATATATATGATTGGAGACTATTATTCCAAGTGAAATAACTCA67360.17168502119555576No Hit
AGATAAAGCAGTTCCTTAGTATATATGGAGCTATTTAATTCAAAGCAGTA66230.16880491320934765No Hit
TAGTTATATCTTGAATTATTTAGTTAAATATCCTAACTTTTTTTTCCCAT65430.1667658987058375No Hit
GTTATATCTTGAATTATTTAGTTAAATATCCTAACTTTTTTTTCCCATAA64790.16513468710302937No Hit
ATTTAATTCAAAGCAGTAGGATCCAGTTACTATGTGTGCTGGATCCAACT64790.16513468710302937No Hit
GAATGATACAATGGACTTTTGGGACTTGGGGGAAAGAGTTGGAGGGGGGC63230.16115860882118455No Hit
GCTATAAACACACATGTGCATATTTTTCATATAAAGACTCCTTTTCCAAT61100.15572973270558874No Hit
ATATACAAATGGCCATCAAACATATGAAAAAATGCTCAACATCACTAATG60910.1552454667610051No Hit
CATATATCACAGTTTCTTTATCCATTCATTCATTGATGGGCATTTGGGTT60600.15445534864089489No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG60260.15358876747690306No Hit
GTTAATATGATATCAACATGTTTATAAAATTTGTGAAAAATTAACAATTG60190.15341035370784592No Hit
CTTTTATCCCTTGCCCCCCTCCAACTCTTTCCCCCAAGTCCCAAAAGTCC59180.15083609789716435No Hit
GTGTAGAAGTGTTCCCTGATCACCAAATTCAAGCCAACCTCTACTGTTTT58830.14994402905187865No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT58670.14953622615117662No Hit
ATATATATGATTGGAGACTATTATTCCAAGTGAAATAACTCAGGAATGGA58660.14951073846988272No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA58520.14915391093176844No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG58270.14851671889942153No Hit
ATTCTAATTAAGTCTTCAAACCTAAACCAAATTTTACTTAGTAGGCACTT57430.14637575367073585No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG57010.14530527105639301No Hit
AATATATACAAATGGCCATCAAACATATGAAAAAATGCTCAACATCACTA55870.14239967538889103No Hit
GGATACAGGTGGTATTTGCTTACATGAGTAAATTCTTCAGTGGTGATTTG54590.1391372521832748No Hit
CATATTAACAGGTAGAAATTGATCATGGTAGAAAATATTTTACATCATGG54470.13883140000774827No Hit
ATATATGATTGGAGACTATTATTCCAAGTGAAATAACTCAGGAATGGAAA54050.1377609173934054No Hit
GAATATAGTTATATCTTGAATTATTTAGTTAAATATCCTAACTTTTTTTT52350.13342801157344633No Hit
ATATATATATGATTGGAGACTATTATTCCAAGTGAAATAACTCAGGAATG51880.1322300905526341No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC49900.12718352965644644No Hit
GTATGAAACTGCTTTATCAATAAATGCAAACAAAAATGTATGCTGGCAAC49190.12537390428458117No Hit
GTTACTATGTGTGCTGGATCCAACTCATATCAGTTTGCAAGAGTCAATTG48550.12374269268177304No Hit
GGTTTAATGAATATAGTTATATCTTGAATTATTTAGTTAAATATCCTAAC48410.12338586514365876No Hit
CACACATATATCACAGTTTCTTTATCCATTCATTCATTGATGGGCATTTG47910.12211148107896491No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT47730.12165270281567513No Hit
CCATAATGTGATACCACCTTCCTCCTGCAAGAATAGCCGTAATCAAAAAA47030.11986856512510374No Hit
GTCTTAAGAGTTGCCAGCATACATTTTTGTTTGCATTTATTGATAAAGCA45940.11709040786407113No Hit
ATGTGATACCACCTTCCTCCTGCAAGAATAGCCGTAATCAAAAAAATCAG45500.11596894988714056No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA45110.11497493031667934No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG44680.11387896002104264No Hit
CTAATGATCAGGGAAATAGAAATCAAAACCATAATGTGATACCACCTTCC44390.1131398172635202No Hit
AACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGC43810.11166153174847532No Hit
TTCTTTATCCATTCATTCATTGATGGGCATTTGGGTTGGTTTCATGATTT42460.10822069477380192No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT41920.10684435998393256No Hit
GTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGG41790.10651302012711215No Hit
ATATGGAGCTATTTAATTCAAAGCAGTAGGATCCAGTTACTATGTGTGCT40780.10393876431643058No Hit
GTTTTCCATAGTGGCTGTACCAGTTTACATTCCCACCAGCAGTGTAGAAG40440.10307218315243877No Hit
CTTAAAATCTACTCTTTTAGCAAAAATCCTGAATACAAATAATTTTATTA40200.10246047880138572No Hit
ATTATGTTCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGC40160.1023585280762102No Hit
GTAGATATCCATTAGTGGGATTGCTGGATCAAATGGTAGTTCTACTTTTA39930.10177231140645103No Hit
GTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTAGG39860.1015938976373939No Hit
TTCTTATGCCTTTGTGTCTTCACAGCTTGGCTCCCACATATTAGTGAGGA39720.10123707009927962No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA73900.092.6072164
TAACAAG82400.083.4941945
CGGTGCA1800.080.551712
GTAGTAA95200.073.1132051
CGGTGCG3650.069.517232
GGTGCGT3700.068.577813
CAACGCA18850.067.6778649
ACAACGC19450.065.962798
GCACGCA1250.063.7969552
TAGTAAC109850.062.4981542
AACAAGA111650.061.55556
ACGAGCG1650.057.1184842
GCGCCTC2700.056.3861966
GCGATCC2200.056.0107779
GAGCGCC1950.055.7665674
CACAGCG4650.054.5582479
ACACGTG7900.052.2989657
AAACACG19700.051.8883825
AGTAACA134650.051.0948523
CGAGCGC1850.050.9435163
Produced by FastQC (version 0.11.5)