FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3544792
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC99110.2795932737379231No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA74420.20994179630285784No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT66790.18841726115382793No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC45670.12883689649491423No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA41060.11583190212571005No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA85900.0112.339714
AGCACGC15250.0105.08441
TAACAAG97450.099.1737445
TAGTAAC98450.098.902822
GCACGCA17050.094.826322
GAAACAC38300.087.64574
GCTATCG259.395735E-486.983687
AAACACG38800.086.516245
AGTAACA112950.086.334513
CGCACTT20100.081.8800665
CGGTGCA8600.081.775212
CACGCAC20300.079.644773
ACAAGAG129050.074.873597
ACGTGCA21250.073.35472
ACGCACT23400.071.572234
GTAGTAA149900.066.6113361
CAAGAGC129150.064.8813558
AACAAGA152000.063.677516
GAAGCGG9850.063.3108331
TAGAAAC63750.063.1191642
Produced by FastQC (version 0.11.5)