FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037876
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT54330.5234729389638069No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC51220.49350789497011205No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT34740.3347220669906617No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA33010.31805340907777035No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT22700.21871591596683998No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT22030.2122604241739861No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT20830.2006983493211135No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA20310.1956881168848687No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC19530.18817276823050152No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT18870.1818136270614216No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT18320.176514342753855No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC15550.1498252199684741No Hit
CACGTGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA14650.14115366382881964No Hit
GAAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGG14160.13643248326389665No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC13410.12920618648085128No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA13330.12843538149065978No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG13270.12785727774801614No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT13130.126508369015181No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT12930.12458135653970223No Hit
CTTCCTTTATTGTTTCTTTGGCCTGGATTTCTTTTTGGTAACTGTGTCTC12800.12332879843064105No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC11970.11533169665740417No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA11670.11244117794418601No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT11260.10849080236945453No Hit
CAACAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG11160.10752729613171515No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC11150.10743094550794122No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT11130.10723824426039336No Hit
CAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAA11040.1063710886464279No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC10740.10348056993320974No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG10700.10309516743811399No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGT10410.10030099934866979No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAACA100.0070873285145.024441
AGCGTCA100.007091414144.996493
TCTGAAC100.007091414144.996493
TCATATA100.007091414144.996492
CGGTGCA2400.0126.871922
GTAACAA22250.0124.143064
GAGCAGA1600.0122.364371
GGTGCAT2500.0121.797053
AGCACGC5550.0118.8939061
CGGAGAT256.459577E-6116.019541
GAAACAC8750.0115.9971854
CACGCAC5800.0113.7472463
GCACGCA5800.0113.7472462
TTTACAG1850.0113.64593
TAACAAG25300.0110.036865
CAGATAA203.8664712E-4108.747372
AAACACG9350.0108.553525
GACAGGC301.5981504E-596.682951
TAGAAAC11850.093.605332
TAGTAAC30850.090.475922
Produced by FastQC (version 0.11.5)