FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3400490
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA69950.20570564830362684No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT61290.18023873030063314No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG59980.1763863443209655No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA52330.153889586500769No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG52000.1529191381242115No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT46180.13580395766492476No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT45710.1344218039164944No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT43590.12818740828527653No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA40420.11886522236501211No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC39530.11624795250096309No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC37920.11151334072442502No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG37400.10998414934318289No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC37220.10945481386506063No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT36820.10827851280256669No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGGAGTA36760.10810206764319259No Hit
ATATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACT35650.10483783219477194No Hit
CCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAATGGATAC35250.103661531132278No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA35210.1035439010260286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC13700.0118.549591
GCACGCA14600.0111.2286452
CGGTGCA3700.094.0504762
CACGCAC17650.092.418583
CGGCGAA406.127939E-790.606899
GTAACAA82800.087.6446154
GCGGTGC11250.082.495461
TAACAAG88350.081.974815
TAGTAAC89450.081.858262
GTAGTAA91600.080.9753341
AGTAACA101850.072.034573
GAAGCGG8800.067.56221
ACGGCGA554.068521E-665.895938
ACAACGC11750.063.5404938
CGCACTT26800.061.4062425
ACGCACT28250.061.333954
AAACACG25900.059.3413735
CCACTAT16700.058.162038
AGCCGTT1750.057.9884157
CGGTGCC5950.057.266732
Produced by FastQC (version 0.11.5)