FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071683
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC31790.2966362254509962No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA16520.15415006116547525No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC15770.14715172303750268No Hit
AGCCAGGACAACTTAAAATCCAAGGACCAAGTTGTTTCCTCCACAAAAAC12670.11822525877521618No Hit
CTTATACATAATGGATATGAAAGCACAGAAGAGGTGGAGCCAGGACAACT11970.11169347652244181No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAACG9050.056.067487
GTAGTAA7800.054.8480031
GCAACGC9450.054.461318
CAACGCA12450.054.1470959
TTATAGC9850.051.525793
TAGCAAC10100.050.2504046
TTTATAG10450.049.2611962
ATACCGT752.5498426E-548.33616
TAGTAGT2700.045.6507643
TATAGCA12100.043.1429674
CATACCG855.3399446E-542.64955
ACAACGC3750.042.525858
CGTCAGA1053.822872E-641.4309462
ATAGCAA13250.038.8512845
AACACGA6750.036.520616
AGCACGC2600.036.2554631
GCACGCA3000.036.252082
TACCGTG1209.576037E-636.243627
AGTAGAA19300.035.3162041
ACACGAG7000.035.2080887
Produced by FastQC (version 0.11.5)