FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l02_n01_FluB_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l02_n01_FluB_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219890
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA90560.40794814157458253No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC80870.3642973300478853No Hit
ATATATATGTATGTGTGTGTATTAGATGCAGGAGGCAGATAACGGAAGTT54370.24492204568694848No Hit
ACAACAGCCAGCGGAAGACAAGTTTAGTTCCAATGACAAAACAAAGTTCC45090.2031181725220619No Hit
TCATTACATTGTGTCGTCACAAGTCAATTTTGAGTTTTTATATAAATAAG33900.15271026942776442No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA32520.14649374518557226No Hit
ACAACAGCCAACACCACAGCCATCTCAATTGGTTAAATTTACACAATTTT31650.14257463207636414No Hit
AGCACGCACTTGGACACATTCTGTATTCATCACTGCCAGATCAATATTGT31540.14207911202807347No Hit
CTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAAGCT30850.13897084990697736No Hit
CATATATATATATGCCCACATCCGTGCACAAACACACACATTCAACTGTA29660.13361022393001454No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG29160.13135786007414782No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT28680.12919559077251577No Hit
CTTATACATAATGGATATGAAAGCACAGAAGAGGTGGAGCCAGGACAACT28170.12689817963953168No Hit
GTTAAAACTATGTTCAAGTGATTATGTCAGTCATTGTAGGAAGAAAAATA27050.12185288460239022No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG26470.11924014252958481No Hit
GTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATTTATCTAA25910.11671749501101406No Hit
ATTATATGTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATT25470.11473541481785132No Hit
ATATATATATGTATGTGTGTGTATTAGATGCAGGAGGCAGATAACGGAAG25230.11365428016703531No Hit
ATGTAATGATGGCATTGAGTCTTTGAGAACAGTTTTTTACTCCTTTATAC25100.11306866556450995No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA17350.090.690062
AGCACGC18000.087.022121
GTAGTAA108900.083.8390351
CGCACTT18100.081.724275
TAGTAAC115250.079.399532
GTAACAA119650.075.570664
AGTAACA121650.075.162713
AAGAGCG25200.074.214969
ACGCACT23150.073.919754
TAACAAG128600.069.747435
CGGTGCA4300.065.765122
TGCGGCG451.19334174E-464.453575
ACAAGAG137750.064.148197
CAAGAGC139000.062.3718538
TGTACGA350.003569214862.13346145
CTGTCGT17200.061.556341
CACGCAC26350.059.9895133
GCGGTGC9350.058.9454651
CGGCGGC902.0534571E-856.380367
GTCGTCC19050.055.9527973
Produced by FastQC (version 0.11.5)